中国中药杂志

2009, v.34(09) 1082-1085

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RP-HPLC法测定20(S)-原人参二醇药质体含量及包封率
Determination of content and entrapment efficiency of 20(S)-protopanaxadiol in pharmacosomes by RP-HPLC method

韩美华;陈婧;陈士林;王向涛;
HAN Meihua1,CHEN Jing2,CHEN Shilin1,WANG Xiangtao1(1.Chinese Academy of Medical Sciences & Peking Union Medical College Institute of Medicinal Plant Development,Beijing 100193,China;2.Harbin University of Commerce,Life Science and Environment Science Research Center,Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin 150076,China)

摘要(Abstract):

目的:建立20(S)-原人参二醇药质体含量及包封率的测定方法。方法:采用RP-HPLC法。色谱条件:COSMO-SIL 5C18-MS-Ⅱ色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-水(95∶5);流速1.0 mL.min-1;检测波长203 nm;柱温25℃;进样量50μL;采用低温超速离心法分离PPD药质体中的游离药物。结果:在上述色谱条件下豆磷脂和试剂对药物的测定无干扰,20(S)-原人参二醇在0.1~0.5 g.L-1与峰面积的线性关系良好(r=0.999 9),回收率在101.44%~103.11%,日内及日间RSD均小于2%(n=3)。结论:RP-HPLC法可用于20(S)-原人参二醇药质体含量及包封率的测定。
Objective: To establish a RP-HPLC method for content and entrapment efficiency of 20(S)-protopanaxadiol in pharmacosomes.Method: The separation was performed with a COSMOSIL 5 C18-MS-Ⅱ column(4.6 mm×250 mm,5 μm) using methanol-water(95∶5) as the mobile phase and detected at 203 nm.The flow rate was 1.0 mL·min-1 and 50 μL sample solution was injected for each time.Result: The calibration curve was linear within the range 0.1-0.5 mg·mL-1(r=0.999 9),the intra-day RSD and inter-day RSD were less than 2% and the average recovery was between 101.44%-103.11%(n=3).Conclusion: The method is simple,accurate,sensitive and applicable for determination of content and entrapment efficiency of 20(S)-protopanaxadiol pharmacosomes.

关键词(KeyWords): 20(S)-原人参二醇;药质体;包封率;反相高效液相色谱法
20(S)-protopanaxadiol;pharmacosomes;entrapment efficiency;RP-HPLC

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基金项目(Foundation): 国家自然科学基金项目(30600787);; 中央级公益性科研院所基本科研业务专项药植所重点项目(YZ-1-23)

作者(Author): 韩美华;陈婧;陈士林;王向涛;
HAN Meihua1,CHEN Jing2,CHEN Shilin1,WANG Xiangtao1(1.Chinese Academy of Medical Sciences & Peking Union Medical College Institute of Medicinal Plant Development,Beijing 100193,China;2.Harbin University of Commerce,Life Science and Environment Science Research Center,Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin 150076,China)

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