中国中药杂志

2019, v.44(03) 535-540

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

葛根素对废用性骨质疏松大鼠模型的防治作用及机制研究
Preventive effect and mechanism of puerarin on rat models of disuse osteoporosis

李凯;秦荣;邵佳乐;高玉海;周建;陈克明;
LI Kai;QIN Rong;SHAO Jia-le;GAO Yu-hai;ZHOU Jian;CHEN Ke-ming;Gansu University of Chinese Medicine;Department of Orthopedics, People′s Liberation Army Joint Service Secuity Forces.No.940 Hospital;

摘要(Abstract):

探讨葛根素(puerarin,Pur)对废用性骨质疏松(disuse osteoporosis,DOP)大鼠模型的防治作用及可能机制。2月龄健康Wistar雌性大鼠30只,随机数字表法分组为对照组(Control)、尾吊组(HLS)、尾吊时服用葛根素组(HLS+Pur),每组10只。通过尾巴悬吊法建立废用性骨质疏松模型,HLS+Pur组每天给予15.4 mg·kg~(-1)葛根素混悬液,Control组和HLS组分别灌服等体积蒸馏水。28 d后,腹主动脉采血处死大鼠,摘取大鼠主要脏器,剥离大鼠骨组织,计算大鼠各器官系数并镜下观察脏器组织病理学情况,进行骨密度检测和骨生物力学实验,骨组织切片后观察双荧光标记和VG染色结果,测定血清骨代谢生化指标。各组大鼠器官系数无统计学意义,脏器病理学检测未见明显异常;骨密度结果显示,葛根素能大幅度提升尾吊引起的胫骨和椎骨的骨密度下降;骨生物力学参数结果表明,葛根素能有效增加胫骨和椎骨的最大载荷和弹性模量;荧光标记观察发现,葛根素用药期间,荧光间距变大,骨形成增强;VG染色结果证明,与HLS组相比,葛根素组骨小梁数目增多、骨小梁厚度变宽但骨分离度减小,极大地改善了尾吊之后的骨微结构;此外血清生化指标显示,葛根素能促使骨形成指标骨钙素(osteocalcin,OC)含量明显增加,同时显著抑制骨吸收指标抗酒石酸酸性磷酸酶5b(tartrate-resistant acid phosphatase 5b,TRACP 5b)的生成。葛根素对废用性骨质疏松大鼠模型具有防治作用且效果良好,其作用机制可能与促进骨形成和抑制骨吸收有关。
To investigate the preventive effect and possible mechanism of puerarin(Pur) in rat model of disuse osteoporosis(DOP),thirty healthy Wistar female rats of 2 months old were randomly divided into control group(Control), hindlimb suspension group(HLS), and puerarin group(HLS+Pur) in hindlimb suspension, with 10 rats in each group. A disuse osteoporosis model was established by tail suspension method, and 15.4 mg·kg~(-1) puerarin suspension was administered to HLS+Pur group every day, and the same volume of distilled water was administered to Control group and HLS group respectively. After 28 days, the rats were sacrificed by abdominal aorta blood collection, the main organs of the rats were removed, and the bone tissues of the rats were dissected. The organ index of the rats was calculated and the histopathology of the organs was observed under microscope. Bone mineral density test and bone biomechanical experiment were performed. Bone histomorphometry results were observed after bone tissue sectioning, and serum biochemical markers of bone metabolism were determined. There was no significant difference in organ index between the groups. There was no obvious abnormality in the pathological examination of the organs. The results of bone mineral density showed that puerarin could significantly increase the bone density of the tibia and vertebrae caused by hindlimb suspension. The mechanical parameters experiments showed that puerarin could effectively increase the maximum load and elastic modulus of the tibia and vertebrae. Fluorescence labeling showed that the fluorosis interval increased and the bone formation increased during puerarin treatment. The VG staining results showed that compared with the HLS group, in the puerarin group, the number of trabecular bone increased, the thickness of the trabecular bone became thicker, and the bone separation became smaller, which greatly improved the bone microstructure after hindlinb suspension. In addition, serum biochemical indicators showed that puerarin could promote bone formation index bone calcium. The content of osteocalcin(OC) increased and inhibited the formation of tartrate-resistant acid phosphatase 5 b(TRACP 5 b). Puerarin has a preventive effect in the rat model of disuse osteoporosis and its effect is good, and its mechanism may be related to promoting bone formation and inhibiting bone resorption.

关键词(KeyWords): 葛根素;废用性骨质疏松;防治作用;机制研究
puerarin;disuse osteoporosis;prevention and treatment effect;mechanism study

Abstract:

Keywords:

基金项目(Foundation): 国家国际科技合作专项(2015DFR30940);; 国家自然科学基金面上项目(81471090,81770879)

作者(Author): 李凯;秦荣;邵佳乐;高玉海;周建;陈克明;
LI Kai;QIN Rong;SHAO Jia-le;GAO Yu-hai;ZHOU Jian;CHEN Ke-ming;Gansu University of Chinese Medicine;Department of Orthopedics, People′s Liberation Army Joint Service Secuity Forces.No.940 Hospital;

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享