王石;董宪喆;谭潇;王羽凝;刘屏;
WANG Shi;DONG Xian-zhe;TAN Xiao;WANG Yu-ning;LIU Ping;Department of Clinical Pharmacology,General Hospital of PLA;

• 1:中国人民解放军总医院临床药理研究室

摘要(Abstract)：

该研究观察抗抑郁中药古方开心散(KXS)在体内外对单胺氧化酶(MAO)活性的影响,从代谢的角度探索KXS升高单胺类神经递质水平的机制。通过在体外酶反应体系和神经胶质细胞C6中分别观察不同浓度的KXS对MAO-A和MAO-B活性的影响;在动物水平的研究中,分别观察不同浓度的KXS灌胃给药1周、2周和3周后,对正常大鼠和孤养结合慢性不可预见性中等强度应激(CMS)模型大鼠脑线粒体MAO-A和MAO-B的活性的影响。结果显示,10g·L-1的KXS在酶反应体系中能显著降低MAO-A和MAO-B的活性;在C6细胞中,0.625¹0 g·L-1的KXS对MAOA的活性没有显著影响,但对MAO-B的活性有较明显的抑制作用,且呈浓度依赖关系;KXS作用1,2,3周对正常大鼠脑内MAO-A和MAO-B均没有显著地影响;338 mg·kg-1剂量给药2周和3周后,CMS大鼠脑内MAO-A活性较模型组均有所降低(P<0.05),而KXS作用1,2,3周对MAO-B没有显著地影响。结果表明开心散在体外对MAO-A和MAO-B的活性均有一定的影响,在体内对正常大鼠脑内MAO-A和MAO-B的活性没有影响,对CMS大鼠脑内MAO-A有一定抑制作用。
To observe the effect of antidepressant medicine prescription,Kaixinsan( KXS) on monoamine oxidase( MAO) activity,and explore the mechanism of KXS in elevating the levels of monoamine neurotransmitter from the perspective of metabolism,in vitro enzyme reaction system and C6 neuroglial cells,the effect of KXS at different concentrations on MAO-A and MAO-B activity was observed. In animal studies,the effect of KXS at different concentrations on MAO-A and MAO-B activities of brain mitochondrialin normal rats and solitary chronic unpredictable moderate stress( CMS) model rats after intragastric administration for 1,2,3 weeks. Results showed that 10 g·L- 1KXS could significantly reduce the activity of MAO-A and MAO-B in enzyme reaction system; and in C6 cells,KXS within 0. 625-10 g·L- 1concentration range had no significant effect on the activity of MAO-A,but had obvious inhibitory effect on the activity of MAO-B in a dose dependent manner. KXS had no significant effect on the activity of MAO-A and MAO-B in brains of normal rats after action for 1,2,3 weeks. After 2 and 3 weeks treatment with 338 mg·kg- 1dose KXS,MAO-A activity in the brain of CMS rats was decreased as compared with the model group( P < 0. 05),while KXS had no significant effect on MAO-B activity after 1,2,3 weeks of treatment. The results indicated that KXS had certain effect on in vitro MAO-A and MAO-B activity,had no effect on brain MAO-A and MAO-B activity in vivo in normal rats,and had certain inhibitory effect on MAO-A activity in brains of CMS rats.

关键词(KeyWords)： 开心散;单胺氧化酶;代谢;慢性应激
Kaixinsan;monoamine oxidase;metabolism;chronic stress

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81302909)

作者(Authors): 王石;董宪喆;谭潇;王羽凝;刘屏;
WANG Shi;DONG Xian-zhe;TAN Xiao;WANG Yu-ning;LIU Ping;Department of Clinical Pharmacology,General Hospital of PLA;

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