朱邵晴;朱振华;郭盛;赵玉洋;鲁学军;沙秀秀;钱大玮;段金廒;
ZHU Shao-qing;ZHU Zhen-hua;GUO Sheng;ZHAO Yu-yang;LU Xue-jun;SHA Xiu-xiu;QIAN Da-wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine;

• 1:南京中医药大学江苏省中药资源产业化过程协同创新中心中药资源产业化与方剂创新药物国家地方联合工程研究中心

摘要(Abstract)：

采用GC-MS和UPLC-TQ-MS技术分析评价不同干燥方法对薄荷药材中2种单萜类、4种酚酸类、5种黄酮类化学成分的组成及含量变化,考察干燥方法对其化学成分的影响;利用TOPSIS综合分析法进行综合评价,为薄荷药材适宜干燥方法的确定提供依据。结果显示,干燥方法对薄荷醇、咖啡酸、迷迭香酸含量影响最大,其次为绿原酸、香叶木素-7-O-葡萄糖苷;同温度下,热风干燥对活性成分的保留作用优于微波干燥与红外干燥;低温(40⁴5℃)干燥对活性成分总量的保留显著高于高温(6045℃)干燥对活性成分总量的保留显著高于高温(60⁷0℃)干燥;微波杀青处理样品酚酸类化学成分总量显著高于未杀青样品,表现出一定的杀酶作用;TOPSIS评价结果显示,薄荷药材产地加工最适干燥方法为热风变温4570℃)干燥;微波杀青处理样品酚酸类化学成分总量显著高于未杀青样品,表现出一定的杀酶作用;TOPSIS评价结果显示,薄荷药材产地加工最适干燥方法为热风变温45⁶0℃干燥。该研究为薄荷药材产地适宜干燥加工方法的确定提供了依据,也为富含挥发油药材的产地加工共性技术形成提供了有益的探索和借鉴。
To provide a scientific basis for the selection of the appropriate drying method for Mentha Haplocalyx Herba( MHH),determine 2 monoterpenes,4 phenolic acids and 5 flavonoids in MHH by GC-MS and UPLC-TQ-MS methods,and investigate the effects of the drying methods on the changes in contents of these analytes. The qualities of products obtained with different drying methods were evaluated by the multivariate statistical method of Technique for Order Preference by Similarity to Ideal Solution( TOPSIS).Results showed that the drying methods had the greatest impact on menthol,caffeic acid,and rosemary acid,which were followed by chlorogenic acid and diosmetin-7-O-glucoside. The contents in these analytes processed with hot-air-drying method were higher than those with microwave-drying and infrared-drying methods at the same temperatures. The contents in these analytes processed under low temperature( 40-45 ℃) were higher than those under higher temperature( 60-70 ℃). Above all,the contents in phenolic acids processed with microwave fixation( exposed under microwave at 100 ℃ for several minutes) were obviously higher than those of not being processed,showing an inhibition of some enzymes in samples after fixation. The TOPSIS evaluation showed that the variable temperature drying method of 'Hot-Air 45-60 ℃ ' was the most suitable approach for the primary drying processing of MHH. The results could provide the scientific basis for the selection of appropriate drying method for MHH,and helpful reference for the primary drying process of herbs containing volatile chemical components.

关键词(KeyWords)： 薄荷;干燥方法;GC-MS;UPLC-TQ-MS;TOPSIS综合评价
Mentha haplocalyx;drying method;GC-MS;UPLC-TQ-MS;TOPSIS evaluation

Abstract:

Keywords:

基金项目(Foundation): 公益性行业科研专项(201407005);; 江苏高校优势学科建设工程项目(ysxk-2014)

作者(Authors): 朱邵晴;朱振华;郭盛;赵玉洋;鲁学军;沙秀秀;钱大玮;段金廒;
ZHU Shao-qing;ZHU Zhen-hua;GUO Sheng;ZHAO Yu-yang;LU Xue-jun;SHA Xiu-xiu;QIAN Da-wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine;

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