黄思敏;吴雨蒙;张丽;谢慧慧;杨硕;徐彭;刘波;
HUANG Si-min;WU Yu-meng;ZHANG Li;XIE Hui-hui;YANG Shuo;XU Peng;LIU Bo;School of Pharmacy, Jiangxi University of Traditional Chinese Medicine;

• 1:江西中医药大学药学院

摘要(Abstract)：

采用碘乙酸钠膝关节腔注射复制大鼠骨关节炎模型,探究甘草附子汤对大鼠骨关节炎防治作用及关节软骨蛋白质组的影响。将60只体质量230～250 g的SD大鼠随机分为正常组、模型组、硫酸氨基葡萄糖组以及甘草附子汤高、中、低剂量组。除正常组外其余各组均进行碘乙酸钠(每侧腿给药3 mg)膝关节腔注射诱导骨关节炎模型。造模后,各给药组灌胃1个月,给药期间每周进行关节疼痛测试、关节宽度测量,观察大鼠自主行为。应用酶联免疫吸附测定(ELISA)法检测大鼠关节灌洗液中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶组织抑制因子(TIMP-1)的含量;应用苏木精-伊红(HE)染色法观察骨关节形态;应用Nano-LC-LTQ-Orbitrap系统检测关节软骨蛋白。结果显示,与模型组比较,甘草附子汤能显著改善骨关节炎大鼠的关节疼痛和关节肿胀;显著降低关节腔灌洗液中TNF-α,IL-1β,MMP-3的含量,升高TIMP-1的含量;减轻关节腔间隙变大、软骨边缘破裂粗糙、软骨层厚薄不一、软骨细胞排列紊乱等炎性病变;各组之间的蛋白进行比较后筛选出差异蛋白273个,KEGG分析发现上述差异蛋白共涉及systemic lupus erythematosus等43条信号通路,其中与骨关节炎相关的信号通路有11条。以上结果表明,甘草附子汤对骨关节炎有防治作用,其作用机制可能是通过调节骨关节炎相关信号通路的蛋白表达来完成的。
The rat osteoarthritis model was replicated by injection of sodium iodoacetate into the knee joint cavity, and the effects of Gancao Fuzi Decoction on rat osteoarthritis and the proteome of articular cartilage were investigated. Sixty SD rats weighing 230-250 g were randomly divided into normal group, model group, glucosamine sulfate group, and Gancao Fuzi Decoction high, medium and low dose groups. Osteoarthritis model was induced by intra-articular injection of sodium iodoacetate(3 mg on each leg) in all groups except the normal group. After modeling, each administration group was given intragastric administration for 1 month. During the administration period, joint pain test and joint width measurement were performed every week to observe the autonomous behavior of rats. Enzyme linked immunosorbent assay(ELISA) method was used to detect the contents of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), matrix metalloproteinase-3(MMP-3), and matrix metalloproteinase tissue inhibitor(TIMP-1) in rat joint lavage fluid. Hematoxylin-eosin(HE) staining was used to observe bone and joint morphology. Nano-LC-LTQ-Orbitrap system was used to detect arti-cular cartilage proteins. The results showed that, compared with the model group, Gancao Fuzi Decoction could significantly improve joint pain and joint swelling in osteoarthritis rats, significantly reduce the contents of TNF-α, IL-1β and MMP-3 in the joint cavity la-vage fluid, increase the content of TIMP-1, and relieve inflammatory diseases such as enlarged joint space, rough cartilage edge, different thickness of cartilage layer, and disordered arrangement of chondrocytes. After comparing the proteins between the groups, 273 differential proteins were screened out. KEGG analysis found that the above differential proteins involved 43 signaling pathways such as systemic lupus erythematosus, among which 11 signaling pathways were related to osteoarthritis. The above results indicated that Gancao Fuzi Decoction had a preventive effect on osteoarthritis, and its mechanism of action may be accomplished by regulating the protein expression of osteoarthritis-related signal pathways.

关键词(KeyWords)： 甘草附子汤;骨关节炎;蛋白质组学;碘乙酸钠
Gancao Fuzi Decoction;osteoarthritis;proteomics;sodium iodoacetate

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81860802,81560723);; 江西中医药大学研究生创新基金项目(JZYC19S11)

作者(Author): 黄思敏;吴雨蒙;张丽;谢慧慧;杨硕;徐彭;刘波;
HUANG Si-min;WU Yu-meng;ZHANG Li;XIE Hui-hui;YANG Shuo;XU Peng;LIU Bo;School of Pharmacy, Jiangxi University of Traditional Chinese Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20201210.401

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