中国中药杂志

2010, v.35(20) 2712-2717

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一测多评法测定黄芪中4种异黄酮的含量
Simultaneous assay of four isoflavonoids in Astragali Radix by QAMS

蔡海霞;陈君;李萍;
CAI Haixia,CHEN Jun,LI Ping(Key Laboratory of Modern Chinese Medicines(Ministry of Education),China Pharmaceutical University,Nanjing 210009,China)

摘要(Abstract):

目的:克服中药多组分含量测定时对照品缺乏的问题,建立黄芪中4个主要异黄酮类化合物的一测多评含量测定方法。方法:以芒柄花素为内参物,建立芒柄花素与毛蕊异黄酮苷、芒柄花苷和毛蕊异黄酮的相对校正因子,并用该校正因子进行毛蕊异黄酮苷、芒柄花苷和毛蕊异黄酮的含量计算(计算值),实现一测多评;同时采用外标法测定药材中毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮和芒柄花素的含量(实测值),并比较计算值与实测值的差异。结果:34批黄芪药材及饮片中4种异黄酮的含量计算值与实测值间无显著差异。结论:以外标法测定芒柄花素,利用相对校正因子实现对毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮含量测定进行质量评价是准确的、可行的。
Based on the complexity of the chemical constitutions in traditional Chinese medicines(TCMs),multi-components analysis used for the quality control of TCMs is more scientific and reasonable.However,routine quality control of TCMs by multi-components analysis is limited for the shortage of various chemical reference substances.Herein,the method of quantitative analysis of multi-components by single marker(QAMS) was validated and used to simultaneously determine calycosin-7-O-β-D-glucopyranoside,ononin,calycosin,formononetin in Radix Astragali.Using formononetin as the internal reference substance,the relative correction factors(RCFs) of the other three isoflavonoids were calculated by HPLC-DAD with good reproducibility in different instruments and chromatographic columns.The other three isoflavonoids in 34 batches of samples were simultaneously calculated according to their RCFs.The results of QAMS method were not significantly different from that of external standard method(RSD<3.5%).It is accurate and feasible to evaluate the quality of Radix Astragali by QAMS.

关键词(KeyWords): 一测多评;校正因子;高效液相色谱法;黄芪;异黄酮
quantitative analysis of multi-components by single marker(QAMS);relative correction factor(RCF);HPLC;Radix Astragali;isoflavonoids

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基金项目(Foundation): 中医药行业科技专项(200707009)

作者(Author): 蔡海霞;陈君;李萍;
CAI Haixia,CHEN Jun,LI Ping(Key Laboratory of Modern Chinese Medicines(Ministry of Education),China Pharmaceutical University,Nanjing 210009,China)

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