汪晋;张玉峰;邵青;范骁辉;
WANG Jin,ZHANG Yufeng,SHAO Qing,FAN Xiaohui(Pharmaceutical Informatics Institute,Zhejiang University,Hangzhou 310058,China)

• 1:浙江大学药物信息学研究所

摘要(Abstract)：

目的:运用代谢组学方法研究结扎冠状动脉致大鼠急性心肌缺血和三七总皂苷给药后大鼠血清代谢物组变化。方法:通过GC-MS技术分析正常组、急性心肌缺血造模组和三七总皂苷给药组的大鼠血清代谢物组,进而使用主成分分析(PCA)和偏最小二乘法-判别(PLS-DA)法研究3组代谢物谱的差异。结果:PCA和PLS-DA模型对不同组大鼠血清代谢物组预测分类效果良好;采用t检验和变量重要性投影法(VIP)挑选得到血清中10种潜在代谢标志物。结论:代谢组学方法可整体上反映生物体的病理生理状态,有望用于中药药效评价。
Objective:The effect of panax notoginosides on ischemic rat after acute myocardial infarction by coronary artery ligation was preliminarily investigated using metabonomics approach.Method: GC-MS based metabonomics approach was applied to analyse the serum metabolome of normal group,model group and treatment group of rats.Furthermore,PCA and PLS-DA was used to identify the differentially expressed metabolites among the three groups.Result: A good fit and prediction results using metabolome data was obtained by PCA and PL-DA.Moreover,10 metabolites were selected as potential biomarkers for efficacy evaluation of panax notoginosides by t-test and variable importance in the projection.Conclusion: Metabonomics provides a whole-organism biological description of pathological or physiological state,and likely can be used as a new approach for efficacy evaluation of traditional Chinese medicine.

关键词(KeyWords)： 气相色谱-质谱;代谢组学;三七总皂苷;急性心肌缺血
gas chromatography-mass spectrometry(GC-MS);metabolomics;panax notoginosides;acute myocardial ischemia

Abstract:

Keywords:

基金项目(Foundation): 国家科技重大专项课题(2009ZX09311-002,2009ZX09502-005);; 浙江省杰出青年团队项目(R2080693)

作者(Authors): 汪晋;张玉峰;邵青;范骁辉;
WANG Jin,ZHANG Yufeng,SHAO Qing,FAN Xiaohui(Pharmaceutical Informatics Institute,Zhejiang University,Hangzhou 310058,China)

参考文献(References)：

• [1]Price K E,Vandaveer S S,Lunte C E,et al.Tissue targetedmetabonomics:Metabolic profiling by microdialysis sampling andmicrocoil NMR[J].J Pharmaceut Biomed,2005,38(5):904.
• [2]Huang X,Shao L,Gong W,et al.A metabonomic characteriza-tion of CCl4-induced acute liver failure using partial least squareregression based on the GC/MS metabolic profiles of plasma inmice[J].J Chromat B,2008,870(2):178.
• [3]Wilson I D,Plumb R,Granger J,et al.HPLC-MS-based methods forthe study of metabonomics[J].J Chromat B,2005,817(1):67.
• [4]Wang S F,Zhao X P,Mao Y,et al.Novel approach for develo-ping urinary nucleosides profile by capillary electrophoresis-massspectrometry[J].J Chromat A,2007,1147(2):254.
• [5]Chan P,Thomas G N,Tomlinson B.Protective effects of trilino-lein extracted fromPanax notoginsengagainst cardiovascular dis-ease[J].Acta Pharm Sin,2002,23(12):1157.
• [6]Fuminari Yamaguchi,Atsushi Sanbe,Satoshi Takeo.Cardiac sarco-plasmic reticular function in rats with chronic heart failure followingmyocardial infarction[J].J Mol Cel C,1997,29(2):753.
• [7]Yang,J,Zhao X J,Liu X L,et al.High performance liquid chro-matography-mass spectrometry for metabonomics:Potential biomar-kers for acute deterioration of liver function in chronic hepatitis B[J].J Proteome Res,2006,5(3):554.