于梦婷;童黄锦;毛春芹;苏丹;殷放宙;费程浩;王梦;季德;陆兔林;
YU Meng-ting;TONG Huang-jin;MAO Chun-qin;SU Dan;YIN Fang-zhou;FEI Cheng-hao;WANG Meng;JI De;LU Tu-lin;School of Pharmacy,Jiangxi University of Traditional Chinese Medicine;School of Pharmacy,Nanjing University of Traditional Chinese Medicine;

• 1:江西中医药大学药学院
• 2:南京中医药大学药学院

摘要(Abstract)：

利用分光测色仪测定不同基原莪术饮片粉末L~*,a~*,b~*,通过SPSS 21.0进行判别分析建立不同基原莪术饮片颜色范围及数学预测模型,广西莪术L~*,a~*,b~*分别在58.09～62.40,4.53～5.66,23.61～24.29,蓬莪术L~*,a~*,b~*分别在64.02～70.71,-0.89～4.13,44.59～54.52,温莪术L~*,a~*,b~*分别在68.55～70.99,-0.11～1.47,28.26～32.19;所建立的数学预测模型通过原始和交叉验证及外部样品验证表明可实现不同基原莪术100%识别。建立一种双波长HPLC同时测定9种倍半萜类化合物及3种姜黄素类成分含量的方法并测定不同基原莪术的含量,结果表明广西莪术中新莪术二酮、异莪术烯醇、β-榄香烯含量较高,蓬莪术中莪术烯醇、呋喃二烯酮、去甲氧基姜黄素、姜黄素含量较高,温莪术以莪术二酮、呋喃二烯、吉马酮3种成分为主。对L~*,a~*,b~*与12种成分含量进行Pearson相关性分析,结果表明莪术烯醇、呋喃二烯酮、去甲氧基姜黄素、姜黄素与b~*(蓝黄轴)有显著正相关(P<0.01),新莪术二酮、β-榄香烯、异莪术烯醇与L~*(亮度)呈显著负相关(P<0.01),莪术二酮、呋喃二烯、吉马酮与L~*有显著正相关(P<0.01),表明莪术的外观颜色可反映内在成分含量的变化。该研究为不同基原莪术饮片的快速识别及质量评价体系的建立提供参考。
L~*, a~* and b~* values of prepared slices of Curcumae Rhizoma were measured by spectrophotometer. SPSS 21.0 was used for discriminant analysis to establish the color range and mathematical prediction model of prepared slices of Curcumae Rhizoma. The values of L~*, a~* and b~* of kwangsiensis ranged from 58.09-62.40, 4.53-5.66 and 23.61-24.29, while the values of L~*, a~* and b~* of phaeocaulis were between 64.02-70.71,-0.89-4.13 and 44.59-54.52, respectively. The values of L~*, a~* and b~* of wenyujin were 68.55-70.99,-0.11-1.47 and 28.26-32.19, respectively. The mathematical prediction model was proved to be able to realize 100% identification of Curcumae Rhizome of different origins through original and cross validation and external samples validation. A dual wavelength HPLC was established; the contents of 9 sesquiterpenoids and 3 Curcumae Rhizomes were determined simultaneously; and the contents of Curcumae Rhizome of different origins were determined. The results showed that kwangsiensis had higher contents of neocurdione, β-elemene and isocurcumaenol, phaeocaulis curcumin, furadienone, demethoxycurcumin and curcumin; and wenyujin mainly contained curdione, furadienes and guimarone. Pearson correlation analysis on L~*, a~*, b~* value and content of 12 components showed that curcumin, furadienone, demethoxycurcumin and curcumin had a significant positive correlation with b~* value(P<0.01). There was a significant negative correlation between neocurdione, β-elemene and isocurcumaenol and L~* value(P<0.01). Curdione, furadienes and guimarone were significantly correlated with L~* value(P<0.01),indicating that the appearance co-lor of Curcumae Rhizoma could reflect the change of the content of the internal components. This study provided reference for the rapid recognition of Curcumae Rhizoma and the establishment of quality evaluation system.

关键词(KeyWords)： 莪术;颜色量化;含量测定;质量识别;相关性研究
Curcumae Rhizoma;color quantization;content determination;quality identification;correlation study

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2018YFC1707000);; 国家自然科学基金项目(81673598,81973483)

作者(Author): 于梦婷;童黄锦;毛春芹;苏丹;殷放宙;费程浩;王梦;季德;陆兔林;
YU Meng-ting;TONG Huang-jin;MAO Chun-qin;SU Dan;YIN Fang-zhou;FEI Cheng-hao;WANG Meng;JI De;LU Tu-lin;School of Pharmacy,Jiangxi University of Traditional Chinese Medicine;School of Pharmacy,Nanjing University of Traditional Chinese Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20201111.301

参考文献(References)：

• [1] 国家药典委员会.中华人民共和国药典.一部[S].北京：中国医药科技出版社，2015:274.
• [2] 王颖，郭兰萍，黄璐琦，等.姜黄、莪术、郁金的化学成分与药理作用研究进展[J].中国药房，2013,24(35):3338.
• [3] 尹国平，张清哲，安月伟，等.温郁金化学成分及药理活性研究进展[J].中国中药杂志，2012,37(22):3354.
• [4] YANG F Q,WANGY T,LI S P,et al.Simultaneous determination of 11 characteristic components in three species of Curcuma Rhizomes using pressurized liquid extraction and high-perfor-mance liquid chromatography [J].J Chromatogr A,2006,1134:226.
• [5] 张建逵，李云静，孙智，等.基于断面色泽测定的条芩饮片分级研究[J].中国中药杂志，2017,42(21):4115.
• [6] 张喜锋，蒋传中，王鹏，等.丹参饮片炮制质量标准研究[J].西北药学杂志，2008,23(6):367.
• [7] 严俊，林刚，赖国亮，等.测色技术在炒青绿茶品质评价中的应用研究[J].食品科学，1996,17(7):21.
• [8] SLLAZZO M S,BACCELLONI C D,BELLINCONTRO A,et al.Combining color chart,colorimetric measurement and chemical compounds for postharvest quality of white wine grapes [J].J Sci Food Agric,2018,98:3532.
• [9] 马婷婷，龚慕辛，王智民，等.甘草色泽与有效成分含量的相关性研究[J].中国中药杂志，2017,42(19):3776.
• [10] 戴辉，苏杭，蔡鹏飞，等.五味子醋蒸前后颜色及8种木脂素变化[J].中成药，2019,41(5):1091.
• [11] 何晓凤，阮豪南，王露露，等.鹿茸规格与其颜色及化学成分的相关性研究[J].中国药学杂志，2019,54(15):1226.
• [12] 刘伟，周冰谦，王晓，等.丹参药材粉末色泽与有效成分含量的相关性[J].中华中医药杂志，2019,34(4):1466.
• [13] FEI C,DAI H,WU X,et al.Quality evaluation of raw and processed crataegi fructus by color measurement and fingerprint ana-lysis [J].J Sep Sci,2018,41:582
• [14] 袁琨，严惠民，金尚忠，等.基于D/8条件的颜色测量仪器SCE测量光泽修正模型的设计[J].光学学报，2013,33(11):115.
• [15] 王军，吴泉英，姚庆香，等.利用傅里叶变换和色度学原理分析白光干涉图的形成[J].大学物理实验，2013,26(1):17.
• [16] 陈楚明，吴纯洁，孙灵根，等.中药饮片有关颜色描述客观化表达的构建思路[J].世界科学技术(中医药现代化),2007,9(4):22.
• [17] CHENG N D,BARBANO M,DRAKE M A,et al.Hunter versus cie color measurement systems for analysis of milk-based beve-rages [J].J Dairy Sci,2018,101:4891.
• [18] 吴伯英，李敏.多基原品种莪术化学成分与质量的相关性研究[J].成都中医药大学学报，2012,35(3):48.