中国中药杂志

2011, v.36(04) 443-445

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人肠道菌群对芒果苷体外代谢转化的研究
Metabolism of mangiferin by human intestinal bacteria in vitro

黄慧学;谭珍媛;邓家刚;梁秋云;农玉梅;宋年梅;
HUANG Huixue1,2,TAN Zhenyuan2,DENG Jiagang1*,LIANG Qiuyun2,NONG Yumei1,SONG Nianmei1(1.Faculty of Pharmaceutical Science,Guangxi Traditional Chinese Medical University,Nanning 530001,China; 2.Faculty of Pharmaceutical Science,Guangxi Medical University,Nanning 530021,China)

摘要(Abstract):

目的:研究离体人肠道菌群在体外对芒果苷代谢转化的情况。方法:芒果苷与人肠道菌群在厌氧条件下孵育,通过大孔树脂、制备液相等方法对代谢产物进行分离和纯化,并通过质谱、核磁等手段进行结构鉴定。结果:在与人肠道菌群共同孵育12 h后,芒果苷代谢物生成的量趋于平衡。代谢物经MS,1H和13C鉴定为芒果苷的苷元。结论:在离体条件下,芒果苷可被人肠道菌群代谢,主要代谢物为芒果苷的苷元(1,3,6,7-tetrahydroxyxanthen)。
Objective:To study the metabolism of mangiferin by human intestinal bacteria in vitro.Method:Human intestinal bacteria and mangiferin were incubated under anaerobic conditions in vitro.The metabolite was separated and purified by D101 macroporous resin column and preparation high performance liquid chromatography,and its structure was identified by MS and NMR.Result:After 12 h incubation with human intestinal bacteria,the content of mangiferin metabolite reached the maximum,and it was determined as 1,3,6,7-tetrahydroxyxanthen by MS and NMR.Conclusion:Mangiferin can be metabolized in vitro by human intestinal bacteria into its aglycone(1,3,6,7-tetrahydroxyxanthen).

关键词(KeyWords): 芒果苷;苷元;人肠道菌群;生物转化
mangiferin;aglycone;human intestinal bacteria;metabolism

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30760303);; 广西重点实验室建设基金(0630006-5A)

作者(Authors): 黄慧学;谭珍媛;邓家刚;梁秋云;农玉梅;宋年梅;
HUANG Huixue1,2,TAN Zhenyuan2,DENG Jiagang1*,LIANG Qiuyun2,NONG Yumei1,SONG Nianmei1(1.Faculty of Pharmaceutical Science,Guangxi Traditional Chinese Medical University,Nanning 530001,China; 2.Faculty of Pharmaceutical Science,Guangxi Medical University,Nanning 530021,China)

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