黄慧学;谭珍媛;邓家刚;梁秋云;农玉梅;宋年梅;
HUANG Huixue1,2,TAN Zhenyuan2,DENG Jiagang1*,LIANG Qiuyun2,NONG Yumei1,SONG Nianmei1(1.Faculty of Pharmaceutical Science,Guangxi Traditional Chinese Medical University,Nanning 530001,China; 2.Faculty of Pharmaceutical Science,Guangxi Medical University,Nanning 530021,China)

• 1:广西中医学院药学院
• 2:广西医科大学药学院

摘要(Abstract)：

目的:研究离体人肠道菌群在体外对芒果苷代谢转化的情况。方法:芒果苷与人肠道菌群在厌氧条件下孵育,通过大孔树脂、制备液相等方法对代谢产物进行分离和纯化,并通过质谱、核磁等手段进行结构鉴定。结果:在与人肠道菌群共同孵育12 h后,芒果苷代谢物生成的量趋于平衡。代谢物经MS,1H和13C鉴定为芒果苷的苷元。结论:在离体条件下,芒果苷可被人肠道菌群代谢,主要代谢物为芒果苷的苷元(1,3,6,7-tetrahydroxyxanthen)。
Objective:To study the metabolism of mangiferin by human intestinal bacteria in vitro.Method:Human intestinal bacteria and mangiferin were incubated under anaerobic conditions in vitro.The metabolite was separated and purified by D101 macroporous resin column and preparation high performance liquid chromatography,and its structure was identified by MS and NMR.Result:After 12 h incubation with human intestinal bacteria,the content of mangiferin metabolite reached the maximum,and it was determined as 1,3,6,7-tetrahydroxyxanthen by MS and NMR.Conclusion:Mangiferin can be metabolized in vitro by human intestinal bacteria into its aglycone(1,3,6,7-tetrahydroxyxanthen).

关键词(KeyWords)： 芒果苷;苷元;人肠道菌群;生物转化
mangiferin;aglycone;human intestinal bacteria;metabolism

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30760303);; 广西重点实验室建设基金(0630006-5A)

作者(Authors): 黄慧学;谭珍媛;邓家刚;梁秋云;农玉梅;宋年梅;
HUANG Huixue1,2,TAN Zhenyuan2,DENG Jiagang1*,LIANG Qiuyun2,NONG Yumei1,SONG Nianmei1(1.Faculty of Pharmaceutical Science,Guangxi Traditional Chinese Medical University,Nanning 530001,China; 2.Faculty of Pharmaceutical Science,Guangxi Medical University,Nanning 530021,China)

参考文献(References)：

• [1]广西壮族自治区卫生厅.广西中药材标准[M].桂林:广西科学技术出版社,1992:54.
• [2]邓家刚,曾春晖.芒果叶及芒果苷30年研究概况[J].广西中医学院学报,2003,6(2):44.
• [3]李好文,邓家刚,邓静.芒果苷国外研究进展[J].广西中医学院学报,2003,6(4):62.
• [4]覃骊兰,梁爱武,邓家刚.芒果苷片治疗急性上呼吸道感染30例[J].山东中医杂志,2008,27(9):587.
• [5]邓家刚,王志萍,李学坚,等.芒果苷滴丸成型工艺的研究[J].中成药,2008,30(7):1070.
• [6]韦玮.芒果苷体内外吸收特征及机理的研究[D].南宁:广西中医学院,2010.
• [7]李艳华,杨勃林.改良厌氧培养法[J].黑龙江医药科学,2000,23(2):92.
• [8]Wang H,Ye G,Ma C H,et al.Identification and determination offour metabolites of mangiferin in rat urine[J].J Pharm BiomedAnal,2007,45:793.
• [9]陈昕,周秋丽,王本祥.人参皂苷Rb1在大鼠肠内菌代谢物吸收入血成分的研究[J].药学学报,1999,34(7):481.
• [10]Li Y J,Sui Y J,Dai Y H,et al.LC determination and pharmaco-kinetics study of mangiferin in rat plasma and tissues[J].Chro-matographia,2008,67:957.