中国中药杂志

2008, (17) 2130-2132

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紫金莲药材的质量控制方法的研究
Study on qualitative and quantitatire methods for Caratostigma willmottianum

王爱民;王永林;李勇军;苏红;赵平;
WANG Ai-min,WANG Yong-lin,LI Yong-jun,SU Hong,ZHAO Ping(School of Pharmacy,Guiyang Medical Colledge,Guiyang 550004,China)

摘要(Abstract):

目的:建立紫金莲药材的定性鉴别和定量检测方法。方法:采用TLC法,以紫金莲对照药材和白花丹醌对照品鉴别紫金莲药材;以HPLC测定白花丹醌含量。结果:采用C18色谱柱,以甲醇-水(75∶25)为流动相,检测波长265 nm,供试品色谱中白花丹醌分离良好,白花丹醌进样量在33.6~313.6 ng线性关系良好,r=0.9999,平均加样回收率100.3%,RSD 1.9%。结论:通过研究制定了紫金莲药材TLC和HPLC质量控制方法。
Objective: To establish the qualitative and quantitative methods of Caratostigma willmottianum.Method: The roots of Radix Ceratostigmae were identified by TLC with the authentic medicinal material and plumbagin as the reference.The contents of plumbagin in Radix Ceratostigmae were determined by HPLC.Result: The TLC method was simple and specific.A Diamonsil C18 column was used.The mobile phase was methanol-water(75∶25).Plumbagin in the sawple extract was separated well.The linear range of plumbagin was 33.6-313.6 ng.The average recovery of plumbagin was 100.3% and RSD was 1.9%.Conclusion: The methods can be used for identification and determination of plumbagin in C.willmottianum.

关键词(KeyWords): 紫金莲;薄层色谱;白花丹醌;高效液相色谱法
Caratostigma willmottianum;TLC;plumbagin;HPLC

Abstract:

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基金项目(Foundation): 国家“十一五”科技支撑计划项目子课题(2006BAI06A01-3);; 贵州省中药现代化科技产业研究开发专项基金项目(黔科合中药专字[2006]5042号)

作者(Author): 王爱民;王永林;李勇军;苏红;赵平;
WANG Ai-min,WANG Yong-lin,LI Yong-jun,SU Hong,ZHAO Ping(School of Pharmacy,Guiyang Medical Colledge,Guiyang 550004,China)

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