中国中药杂志

2008, v.33(24) 2935-2937

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HPLC测定大枣中无刺枣苄苷Ⅱ的含量
Determination of zizybeoside Ⅱ of Ziziphus jujuba by HPLC

牛继伟;张启伟;龚慕辛;闫利华;朱晶晶;王智民;李勤凡;
NIU Ji-wei1,2,ZHANG Qi-wei2,GONG Mu-xin2,YAN Li-hua2,ZHU Jing-jing2,WANG Zhi-min2,LI Qin-fan1(1.College of Veterinary Medicine,Northwest Agricultural and Forest University,Yangling 712100,China;2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

摘要(Abstract):

目的:建立大枣中无刺枣苄苷Ⅱ的HPLC分析方法。方法:采用Zorbax SB-C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-水(20∶80)为流动相,流速为1 mL.min-1,检测波长为210 nm,柱温30℃。结果:无刺枣苄苷Ⅱ在0.0460.582μg呈良好的线性关系,r=0.999 9。12批不同产地的大枣中无刺枣苄苷Ⅱ质量分数在0.013%0.582μg呈良好的线性关系,r=0.999 9。12批不同产地的大枣中无刺枣苄苷Ⅱ质量分数在0.013%0.041%。结论:该方法简单、重复性好,可用于大枣的质量控制。
Objective:To develop a quantitative method for determination of zizybeoside Ⅱ in Ziziphus jujuba.Method: The samples were separated at 30 ℃ on a Zorbax SB-C18 column eluted with methanol-water(20∶80) as the mobile phase.Flow rate was set at 1.0 mL·min-1 and the detection wavelength was set at 210 nm.Result: The calibration curve was linear within the range from 0.046 to 0.582 μg(r=0.999 9) and the average recovery was 97.2%.12 batches of the crude drugs purchased from different areas were determined and the contents of zizybeosideⅡ in Fructus Jujubae were fluctuated from 0.013% to 0.041%.Conclusion: The method is simple,repeatable and could be used for the quality control of Z.jujuba.

关键词(KeyWords): 大枣;无刺枣苄苷Ⅱ;HPLC;含量测定
Ziziphus jujuba;zizybeosideⅡ;HPLC;assay

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑计划项目(2006BAI08B03-09)

作者(Authors): 牛继伟;张启伟;龚慕辛;闫利华;朱晶晶;王智民;李勤凡;
NIU Ji-wei1,2,ZHANG Qi-wei2,GONG Mu-xin2,YAN Li-hua2,ZHU Jing-jing2,WANG Zhi-min2,LI Qin-fan1(1.College of Veterinary Medicine,Northwest Agricultural and Forest University,Yangling 712100,China;2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

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