中国中药杂志

2013, v.38(08) 1258-1262

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蒙药五味风湿胶囊对佐剂性关节炎的作用及机制研究
Study on effects and mechanism of traditional Mongolian medicine Wuweifengshi capsule on adjuvant arthritis in rats

王秀兰;霍万学;鲁仁杰;韩斯日古楞;包春霞;
WANG Xiu-lan1*,HUO Wan-xue1,LU Ren-jie2,HAN Serguleng1,BAO Chun-xia1(1.College of Mongolian Medicine and Pharmacy,Inner Mongolian University of Nationality,Tongliao 028300,China; 2.College of Medicine,Inner Mongolian University of Nationality,Tongliao 028300,China)

摘要(Abstract):

目的:观察蒙药五味风湿胶囊对佐剂性关节炎的作用及其免疫调节机制。方法:取Wistar大鼠,分成正常组、佐剂性关节炎(AA)模型组、五味风湿胶囊低剂量组(0.2 g.kg-1.d-1)、中剂量组(0.4 g.kg-1.d-1)、高剂量组(0.8 g.kg-1.d-1)及忠伦-5汤组(原剂型1.68 g.kg-1.d-1),测定各组大鼠关节肿胀程度,关节组织白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、前列腺素(PGE2)及一氧化氮(NO)水平,测定血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)水平。通过细胞培养,考察药物对AA大鼠脾细胞增殖能力的影响,探讨该药干预下AA大鼠腹腔巨噬细胞培养液对C57BL/6J小鼠胸腺细胞增殖能力的影响。结果:五味风湿胶囊可抑制AA大鼠关节组织IL-1β,TNF-α,PGE2,NO水平;提高血清SOD活性,但MDA未见显著变化;可抑制AA大鼠脾细胞增殖能力;抑制AA大鼠腹腔巨噬细胞培养液对C57BL/6J小鼠胸腺细胞增殖能力的影响。结论:五味风湿胶囊具有良好的抗佐剂性关节炎作用。其作用机制可能与抑制相关细胞因子,调节相关酶的活性及免疫器官细胞增殖等有关。
Objective: To study the effects and immunoregulation mechanism of the traditional Mongolian medicine Wuweifengshi capsule on adjuvant arthritis(AA).Method: Wister rats were divided into several groups: normal group,AA model group,Wuweifengshi capsule groups(with low,moderate,high dose of 0.2,0.4,0.8 g·kg-1·d-1 respectively),and Zhonglun-5 group(original dose of 1.68 g·kg-1·d-1).The edema degree,the level of IL-1β,TNF-α,PGE2,NO and MDA and the activity of SOD in serum were detected.Through cell culture,the effects of the medicine on AA rat′s splenic cell′s multiplication capacity were studied.The influence of celiac macrophage cell culture fluid of AA rats′ on C57BL/6J mice thymic cell multiplication capacity under the medicine was evaluated.Result: Wuweifengshi capsule showed an inhibiting function on the level of IL-1β,TNF-α,PGE2,NO and increased the activity of SOD in serum,but showed no significant influence on MDA.It also inhibited the AA rat′s splenic cell′s multiplication capacity and the influence of celiac macrophage cell culture fluid of AA rat′s on C57BL/6J mice thymic cell multiplication capacity.Conclusion: The anti-AA effect of Wuweifengshi capsule is possibly due to its inhibition of relevant cytokines and its adjustment of corresponding enzyme′s activity and immunization organ′s cell multiplication capacity.

关键词(KeyWords): 五味风湿胶囊;佐剂性关节炎;IL-1β;TNF-α;免疫调节
Wuweifengshi capsule;adjuvant arthritis;IL-1β;TNF-α;immunoregulation

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作者(Author): 王秀兰;霍万学;鲁仁杰;韩斯日古楞;包春霞;
WANG Xiu-lan1*,HUO Wan-xue1,LU Ren-jie2,HAN Serguleng1,BAO Chun-xia1(1.College of Mongolian Medicine and Pharmacy,Inner Mongolian University of Nationality,Tongliao 028300,China; 2.College of Medicine,Inner Mongolian University of Nationality,Tongliao 028300,China)

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