中国中药杂志

2013, v.38(02) 179-185

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乳香-没药配伍前后化学成分溶出变化及其对LPS-诱导的巨噬细胞产生NO的影响
Change in dissolution of chemical components of frankincense-myrrh before and after their compatibility and effect on no release of LPS-induced macrophage cells

陈婷;宿树兰;段金廒;尚尔鑫;钱大玮;唐于平;
CHEN Ting,SU Shu-lan*,DUAN Jin-ao*,SHANG Er-xin,QIAN Da-wei,TANG Yu-ping(Key Laboratory for Traditional Chinese Medicine Formulae Research of Jiangsu Province, Nanjing University of Chinese Medicine,Nanjing 210046,China)

摘要(Abstract):

目的:分析乳香-没药配伍前后化学成分的变化,评价差异性化合物对LPS-诱导的小鼠腹腔巨噬细胞产生NO的影响,以期从化学成分变化角度探讨乳香-没药配伍协同增效的物质基础。方法:采用UPLC-Q-TOF-MS/MS联用技术对乳香-没药配伍前后化学成分进行分析,并结合MarkerLynx 4.1统计软件分析2味药配伍后的差异性化合物。结果:经PCA分析乳香-没药合提液与合并液色谱图存在明显差异,提示2味药配伍前后化学成分存在显著差异。经OPLS-DA分析及化学成分鉴定,2味药配伍后五环三萜类化合物(α-乳香酸、β-乳香酸等),四环三萜类化合物(榄香酮酸、3-乙酰氧基-16-羟基-24-甲基达玛烷、3-羟基甘遂烷-7,24-二烯-21-酸/3-羟基甘遂烷-8,24-二烯-21-酸等)溶出量显著增加,而环倍半萜类,大环二萜类化合物溶出量均有下降趋势。体外活性评价表明:化合物2-甲氧基-8,12-环氧-吉玛-1(10),7,11-三烯-6-酮,2-甲氧基-5-乙酰氧基-呋喃-吉玛-1(10)-烯-6-酮,3-羰基甘遂-8,24-二烯-21-羧酸显著抑制LPS-诱导的小鼠腹腔巨噬细胞产生NO水平。结论:该研究结果为揭示乳香-没药配伍增强抗炎效应的物质基础提供了一定科学依据与参考。
Objective: To analyze the difference of chemical compounds of frankincense-myrrh before and after their compatibility,and evaluate the effect of differentiated compounds on NO generated by LPS-induced peritoneal macrophage cells in rats,in order to discuss synergetic material basis of frankincense-myrrh compatibility from the prospective of change in chemical constituents.Method: UPLC-Q-TOF-MS/MS combined technology was used to analyze the chemical components of frankincense-myrrh before and after their compatibility.MarkerLynx 4.1 statistical software was used to analyze differentiated compounds before and after their compatibility.Result: The results of PCA showed that there were significant differences in the combined extracts of frankincense-myrrh and the chromatogram of their combined liquid,suggesting significant differences in their chemical compounds before and after their compatibility;after their compatibility,the dissolution of pentacyclic triterpenoid(α-boswellic acid,β-boswellic acid) and tetracyclic triterpenoid(elemonic acid,3-acetoxy-16-hydroxy-dammar-24-ene,3-hydroxytirucalla-8,24-dien-21-oic acid or 3-hydroxytirucalla-7,24-dien-21-oic acid) increased notably,while the dissolution of both yclic sesquiterpenes and macrocyclic diterpenoids decreased.According to the evaluation on in vitro activity,2-methoxy-8,12-epoxy-germa-1(10),7,11-triene-6-ketone,2-methoxy-5-acetoxyl-furan-germa-1(10)-alkene-6-ketone and 3-carbonyl Euphorbia kansui-8,24-diene-21-carboxylic acid notably inhibited NO generated by LPS-induced peritoneal macrophage cells in rats.Conclusion: These findings provide scientific basis and reference for studies on anti-inflammatory material basis of frankincense-myrrh compatibility.

关键词(KeyWords): 乳香-没药;药对;化学成分溶出变化;巨噬细胞;NO
frankincense-myrrh;herbal pair;change in dissolution of chemical components;macrophage cell;NO

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30973885);; 江苏省高校自然基金重大基础研究项目(11KJA360002,06KJA36022);; 江苏省方剂高技术研究重点实验室建设项目(BM2010576);; 江苏省理血方剂创新药物工程中心建设项目

作者(Author): 陈婷;宿树兰;段金廒;尚尔鑫;钱大玮;唐于平;
CHEN Ting,SU Shu-lan*,DUAN Jin-ao*,SHANG Er-xin,QIAN Da-wei,TANG Yu-ping(Key Laboratory for Traditional Chinese Medicine Formulae Research of Jiangsu Province, Nanjing University of Chinese Medicine,Nanjing 210046,China)

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