中国中药杂志

2007, (14) 1393-1395

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丹参功能基因组学研究Ⅲ——丹参金属硫蛋白MT-2基因的分析
Functional genomics studies of Salvia miltiorrhiza Ⅲ.analyze of metallothionein(MT-2) genes

崔光红;毛莹;黄璐琦;袁媛;王学勇;
CUI Guang-hong1,MAO Ying1,2,HUANG Lu-qi1,YUAN yuan1,WANG Xue-yong1 (1.Institute of Chinese Materia Medica,Academy of Chinese Medical Sciences,Beijing 100700,China;2.College of Chemistry and Pharmacy,Jiamusi University,Jiamusi 154007,China)

摘要(Abstract):

目的:获得丹参金属硫蛋白基因序列,并进行生物信息学分析和初步的表达特性研究。方法:利用cDNA芯片技术,从不同时期毛状根中获得目的基因。利用BLAST进行序列比对,ORF finder寻找开放读码框,Pro-site分析蛋白质的基本结构域。用半定量RT-PCR检测其在丹参组培苗根、茎、叶中的表达情况。结果:得到2个MT基因全长序列,分别编码80和79个氨基酸,具有Type 2型金属硫蛋白的典型特征,分别命名为SmMT-2 a和SmMT-2b,两者具有71.25%的同源性。半定量RT-PCR表明,MT基因在丹参组培苗根、茎、叶中均有转录水平的表达,叶中表达量高于根和茎。结论:首次得到丹参金属硫蛋白基因,为其功能研究提供了基础。
Objective: To study the metallothionein genes of Salvia miltiorrhiza through bioinformatics and characterization of tissue expression in regenerated shoots.Method: Metallothionein genes were obtained by cDNA microarray analyze.BLAST was used for align,ORF finder software was used to find open reading frame,Prosite database was used to analyze the protein.semi-quantitative RT-PCR method was used to detect the gene expression level.Result: Two metallothionein genes were obtained which were contained a deduced amino acid sequence of 80 and 79 residues, named as SmMT-2a and SmMT-2b,they had a homology of 71.25%.Semi-quantitative RT-PCR indicated that metallothionein genes were expressed in all tissues such as root,stem and leaf in regenerated shoots,while the expression level was higher in leaf than in root and stem.Conclusion: It was the first time that metallothionein genes were obtained from S.miltiorrhiza.It provides a good basis for further functional study of S.miltiorrhiza.

关键词(KeyWords): 丹参;金属硫蛋白;表达
Salvia miltiorrhiza;metallothionein

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基金项目(Foundation): 国家重点基础研究发展计划(2006CB504700);; 国家高科技研究发展计划(2003AA2Z2040)

作者(Author): 崔光红;毛莹;黄璐琦;袁媛;王学勇;
CUI Guang-hong1,MAO Ying1,2,HUANG Lu-qi1,YUAN yuan1,WANG Xue-yong1 (1.Institute of Chinese Materia Medica,Academy of Chinese Medical Sciences,Beijing 100700,China;2.College of Chemistry and Pharmacy,Jiamusi University,Jiamusi 154007,China)

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