中国中药杂志

2021, v.46(03) 560-566

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基于稳定同位素指纹的春三七主根产地判别研究
Origin traceability of main root of spring Panax notoginseng based on stable isotope fingerprint

梁社往;何忠俊;熊俊芬;洪常青;
LIANG She-wang;HE Zhong-jun;XIONG Jun-fen;HONG Chang-qing;College of Agronomy and Biotechnology, Yunnan Agricultural University;College of Resource and Environment, Yunnan Agricultural University;

摘要(Abstract):

研究建立春三七主根产地判别技术,为三七地理标志产品保护和溯源提供理论依据。采集广西百色片区、云南文山片区、云南新拓展片区三七主产县三年生春三七样品共44份,利用元素分析-稳定同位素质谱仪测定了其稳定性碳、氮、氢、氧同位素比率,结合Duncan多重比较分析、Fisher判别分析和逐步判别分析建立3个片区三年生春三七主根产地判别模型。3个三七产区地理气候环境具有明显的差异,从广西百色-云南文山-云南新拓展片区,经度、年均温、年降水逐渐降低,海拔、纬度呈增加的趋势。多重比较分析结果表明,3个片区春三七主根δ~(13)C,δ~(15)N,δ~2H,δ~(18)O有显著或极显著差异。Fisher判别分析和逐步判别分析结果表明,基于4种稳定同位素比率的产地判别模型,对3个产地春三七主根正确判别率分别80.05%,76.47%,90.91%,平均为84.09%。利用稳定同位素指纹特征结合化学计量学方法可以实现对春三七主根原料及产品的产地判别。
This paper established the identification technology of the main root origin of three-year-old spring Panax notoginseng aiming at providing theoretical basis for the protection and traceability of geographical indication products of P. notoginseng. Forty-four samples of three-year-old spring P. notoginseng from Guangxi Baise, Yunnan Wenshan, Yunnan new cultivating regions. The stable isotopic ratios of carbon, nitrogen, hydrogen and oxygen were determined by elemental analysis and stable isotope mass spectrometer. Combined with Duncan multiple comparative analysis, fisher discriminant analysis and sequential discriminant analysis, a origin discriminant model for the main root of three-year-old spring P. notoginseng was established for 3 production areas of P. notoginseng. The geographical climate and environment of three production areas of P. notoginseng are obviously different. From Guangxi Baise-Yunnan Wenshan-Yunnan new cultivating regions, the longitude, average annual temperature and annual precipitation gradually decrease, and the elevation and latitude are increasing. The results of multiple comparative analysis showed that there were significant or very signi-ficant differences in the δ~(13)C,δ~(15)N,δ~2H,δ~(18)O of the main roots of P. notoginseng in three regions. The results of fisher′s discriminant analysis and sequential discriminant analysis showed that the correct discriminant rates of the main roots of P. notoginseng for three regions were 80.05%,76.47% and 90.91%, respectively, based on four stable isotope ratios, with an average of 84.09%. Using stable isotope fingerprint and chemometrics method, we can distinguish the origin of the main raw materials and products of P. notoginseng.

关键词(KeyWords): 春三七主根;稳定同位素;判别分析;产地溯源
main root of spring Panax notoginseng;stable isotope;discriminant analysis;origin traceability

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81860679);; 云南省社会发展科技计划项目(2010CA027)

作者(Author): 梁社往;何忠俊;熊俊芬;洪常青;
LIANG She-wang;HE Zhong-jun;XIONG Jun-fen;HONG Chang-qing;College of Agronomy and Biotechnology, Yunnan Agricultural University;College of Resource and Environment, Yunnan Agricultural University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20201121.103

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