梁社往;何忠俊;熊俊芬;洪常青;
LIANG She-wang;HE Zhong-jun;XIONG Jun-fen;HONG Chang-qing;College of Agronomy and Biotechnology, Yunnan Agricultural University;College of Resource and Environment, Yunnan Agricultural University;

• 1:云南农业大学农学与生物技术学院
• 2:云南农业大学资源与环境学院

摘要(Abstract)：

研究建立春三七主根产地判别技术,为三七地理标志产品保护和溯源提供理论依据。采集广西百色片区、云南文山片区、云南新拓展片区三七主产县三年生春三七样品共44份,利用元素分析-稳定同位素质谱仪测定了其稳定性碳、氮、氢、氧同位素比率,结合Duncan多重比较分析、Fisher判别分析和逐步判别分析建立3个片区三年生春三七主根产地判别模型。3个三七产区地理气候环境具有明显的差异,从广西百色-云南文山-云南新拓展片区,经度、年均温、年降水逐渐降低,海拔、纬度呈增加的趋势。多重比较分析结果表明,3个片区春三七主根δ~(13)C,δ~(15)N,δ~2H,δ~(18)O有显著或极显著差异。Fisher判别分析和逐步判别分析结果表明,基于4种稳定同位素比率的产地判别模型,对3个产地春三七主根正确判别率分别80.05%,76.47%,90.91%,平均为84.09%。利用稳定同位素指纹特征结合化学计量学方法可以实现对春三七主根原料及产品的产地判别。
This paper established the identification technology of the main root origin of three-year-old spring Panax notoginseng aiming at providing theoretical basis for the protection and traceability of geographical indication products of P. notoginseng. Forty-four samples of three-year-old spring P. notoginseng from Guangxi Baise, Yunnan Wenshan, Yunnan new cultivating regions. The stable isotopic ratios of carbon, nitrogen, hydrogen and oxygen were determined by elemental analysis and stable isotope mass spectrometer. Combined with Duncan multiple comparative analysis, fisher discriminant analysis and sequential discriminant analysis, a origin discriminant model for the main root of three-year-old spring P. notoginseng was established for 3 production areas of P. notoginseng. The geographical climate and environment of three production areas of P. notoginseng are obviously different. From Guangxi Baise-Yunnan Wenshan-Yunnan new cultivating regions, the longitude, average annual temperature and annual precipitation gradually decrease, and the elevation and latitude are increasing. The results of multiple comparative analysis showed that there were significant or very signi-ficant differences in the δ~(13)C,δ~(15)N,δ~2H,δ~(18)O of the main roots of P. notoginseng in three regions. The results of fisher′s discriminant analysis and sequential discriminant analysis showed that the correct discriminant rates of the main roots of P. notoginseng for three regions were 80.05%,76.47% and 90.91%, respectively, based on four stable isotope ratios, with an average of 84.09%. Using stable isotope fingerprint and chemometrics method, we can distinguish the origin of the main raw materials and products of P. notoginseng.

关键词(KeyWords)： 春三七主根;稳定同位素;判别分析;产地溯源
main root of spring Panax notoginseng;stable isotope;discriminant analysis;origin traceability

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81860679);; 云南省社会发展科技计划项目(2010CA027)

作者(Author): 梁社往;何忠俊;熊俊芬;洪常青;
LIANG She-wang;HE Zhong-jun;XIONG Jun-fen;HONG Chang-qing;College of Agronomy and Biotechnology, Yunnan Agricultural University;College of Resource and Environment, Yunnan Agricultural University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20201121.103

参考文献(References)：

• [1] 崔秀明,杨野,董丽,等.三七栽培学[M].北京:科学出版社,2017.
• [2] 李耿,陈洁,李振坤,等.三七专利数据分析与对产业发展启示[J].中国中药杂志,2020,45(5):1011.
• [3] 吴连举,关一鸣,刘继永.三七无公害栽培技术[M].北京:化学工业出版社,2014.
• [4] 崔秀明,黄璐琦,郭兰萍,等.中国三七产业现状及发展对策[J].中国中药杂志,2014,39(4):553.
• [5] 孙玉琴,向飞军,曾江,等.云南省不同产地三七皂苷含量的比较[J].文山学院学报,2013,26(6):8.
• [6] 杨健,吴浩,杨光,等.基于稳定同位素比和元素分析技术的何首乌产地识别研究[J].中国中药杂志,2018,43(6):2676.
• [7] 廖保生,宋经元,谢彩香,等.道地药材产地溯源研究[J].中国中药杂志,2014,39(20):3881.
• [8] 唐启义,冯光明.DPS数据处理系统[M].北京:科学出版社,2007:625.
• [9] 刘雯雯,陈岩,杨慧,等.稳定同位素及矿物元素分析在谷物产地溯源中应用的研究进展[J].食品科学,2019,40(13):340.
• [10] ZHAO Y,ZHANG B,CHEN G,et al.Recent developments in application of stable isotope analysis on agro-product authenticity and traceability[J].Food Chem,2014,145:300.
• [11] 郭波莉,魏益民,潘家荣.牛肉产地溯源技术研究[M].北京:科学出版社,2009.
• [12] BONTEMPO L,CEPPA F A,PERINI M,et al.Use of δ18O authenticity thresholds to differentiate tomato passata from diluted tomato paste[J].Food Control,2014,35:413.
• [13] FRANCESCA C,SILVIA P,MARCO C,et al.Isoscapes of carbon and oxygen stable isotope compositions in tracing authenticity and geographical origin of Italian extra-virgin olive oils[J].Food Chem,2016,202:291.
• [14] FEDERICA C,MARKUS B,LUANA B,et al.Stable isotope techniques for verifying the declared geographical origin of food in legal cases [J].Trends Food Sci Tech,2017,61:176.
• [15] CHUNG I M,KIM J K,LEE J H,et al.C/N/O/S stable isotopic and chemometric analyses for determining the geographical origin of Panax ginseng cultivated in Korea[J].J Ginseng Res,2017,6:1.
• [16] TIAN Z,DU S,LIU C,et al.Identification of geographical origins of raw American ginseng and tablets based on stable isotope ratios[J].J Chromatogr B,2016,108(10):73.
• [17] 杨健,吴浩,吕朝耕,等.基于稳定同位素的铁皮石斛产地识别研究[J].中国中药杂志,2018,43(6):1118.
• [18] 丁颖,何忠俊,陈中坚,等.生态因子对三七稳定同位素比率的影响[J].云南农业大学学报,2014,29(3):370.
• [19] 何忠俊,梁社往,丁颖,等.三七主根稳定碳同位素组成与生态因子的关系[J].生态环境学报,2015,24(4):561.
• [20] 何忠俊,梁社往,丁颖,等.三七主根稳定氧同位素δ18O与生态因子关系的研究[J].核农学报,2016,30(3):556.
• [21] 石辉,刘世荣,赵晓广.稳定性氢氧同位素在水分循环中的应用[J].水土保持学报,2003,17(2):163.
• [22] ADNAN S,MINE B,AHMET C G.13C/12C pattern of honey from Turkey and determination of adulteration in commercially available honey samples using EA-IRMS[J].Food Chem,2012,130:1115.
• [23] 袁玉伟,张志恒,杨桂玲,等.氮稳定同位素的印迹规律与有机食品鉴别[J].核农学报,2009,23(4):659.
• [24] 李安,陈秋生,赵杰,等.基于稳定同位素与稀土元素指纹特征的大桃产地判别分析[J].食品科学,2020,41(6):322.