中国中药杂志

2021, v.46(06) 1386-1392

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不同年份桔梗转录组学分析及桔梗皂苷生物合成关键基因挖掘
Transcriptome analysis of Platycodon grandiflorum at different growth years and discovery of key genes for platycodin biosynthesis

张健;潘媛;王钰;陈大霞;
ZHANG Jian;PAN Yuan;WANG Yu;CHEN Da-xia;Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica,Chongqing Sub-center of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Science,Chongqing Academy of Chinese Materia Medica;

摘要(Abstract):

桔梗是食药两用大宗中药材品种之一。为探究不同年份桔梗的基因表达差异和主要活性物质桔梗皂苷生物合成过程中关键基因的表达规律,该研究利用Illumina Hiseq 4000测序平台对同一产地一年生、二年生、三年生桔梗进行转录组测序与生物信息学分析。对测序数据进行过滤、组装和拼接后,共获得59 654条unigene,其中1 671条unigene在至少2个样品间差异表达。对差异基因进行聚类分析,结果显示一年生与二、三年生桔梗的基因表达存在较大差异,其中一年生与三年生桔梗之间有1 128个差异基因,而二年生与三年生桔梗之间仅有57个差异基因。KEGG富集结果显示一年生与二、三年生桔梗的差异基因主要集中在倍半萜和三萜的生物合成、萜类骨架的生物合成等代谢途径中。在三萜类生物合成相关通路中,共鉴定15条unigene,涉及5种酶。ACAT,HMGR,FDFT1,SQLE随桔梗生长年份的增加表达量逐渐降低,HMGS在一年生桔梗中表达量最高,其次是三年生。该研究为桔梗发育研究提供了有用数据,也为桔梗皂苷生物合成途径相关基因研究提供了依据。
Platycodon grandiflorum is a medicinal and edible medicinal material. Our study is aimed to explore the differences in the gene expression of P. grandiflorum in different growth years, and the expression rules of key genes in the biosynthesis of the main active substances of P. grandiflorum. Illumina Hiseq 4000 sequencing platform was used to sequence the transcriptome of P. grandiflorum in different years. Then, 59 654 unigenes were obtained through filtering, assembly, splicing and bioinformatics analysis of the sequencing data, of which 1 671 unigenes were differentially expressed between at least two samples. The results of cluster analysis showed that there was a great difference in the gene expression of P. grandiflorum from one-year-old to two/three-year-old. There were 1 128 different genes between one-and three-year old P. grandiflorum, and only 57 different genes between two-and three-year-old P. grandiflorum. KEGG enrichment results showed that the differential genes of P. grandiflorum in different years were mainly concentra-ted in the biosynthesis of sesquiterpenes and triterpenes, and the biosynthesis of terpenoid skeletons. In the triterpenoid biosynthesis-related pathways, a total of 15 unigenes were identified, involving 5 enzymes. The expression levels of ACAT, HMGR, FDFT1, SQLE decreased with the increase of the growth year of P. grandiflorum. The expression of HMGS was the highest in the one-year-old P. grandiflorum, followed by the three-year-old sample. This study provides useful data for the development of P. grandiflorum, and also provides a basis for the study of related genes in the biosynthetic pathway of platycodin.

关键词(KeyWords): 桔梗;转录组;桔梗皂苷;不同年份;表达差异
Platycodon grandiflorum;transcriptome;platycodin;different years;expression difference

Abstract:

Keywords:

基金项目(Foundation): 重庆市技术创新与应用发展专项(cstc2019jsccx-lyjsBX0006);; 重庆英才计划创新领军人才项目(CQYC20200301170);; 重庆市脱贫攻坚专家团项目(渝人社办[2019] 297号)

作者(Author): 张健;潘媛;王钰;陈大霞;
ZHANG Jian;PAN Yuan;WANG Yu;CHEN Da-xia;Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica,Chongqing Sub-center of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Science,Chongqing Academy of Chinese Materia Medica;

Email:

DOI: 10.19540/j.cnki.cjcmm.20201223.103

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