中国中药杂志

2012, v.37(03) 381-383

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LC-MS/MS测定大鼠血浆中香柑内酯及其药代动力学研究
Phamacokinetic study of bergapten in rats plasma by LC-MS/MS

马玉凤;李文;胡芳弟;石义凯;毕映燕;封士兰;
MA Yufeng1,2,LI Wen1,HU Fangdi1,SHI Yikai1,BI Yingyan1,FENG Shilan1,3(1.School of Pharmacy,Lanzhou University,Lanzhou 730000,China; 2.Hanzhong Institute of Food and Drug Control,Hanzhong 723000,China; 3.Key Laboratory of Preclinical Study for New Drugs of Gansu Province,Lanzhou 730000,China)

摘要(Abstract):

目的:建立测定香柑内酯(berg)血浆药物浓度的液相色谱-电喷雾离子化-质谱(LC-ESI-MS)联用的分析方法,并探讨其在大鼠体内的药动学。方法:采用Hypersil ODS柱(4.6 mm×250 mm,5 mm),流动相甲醇-水(77.5∶2.5),流速0.8mL.min-1,柱温30℃,检测波长300 nm。结果:Berg在8.12~162.4μg.L-1(r=0.999 9)线性关系良好。方法学考察均符合要求。日内、日间变异系数(RSD)均<10%,精密度和准确度等均符合生物样品分析的要求。结论:该法准确、灵敏、特异,适用于berg的体内定量分析,berg在大鼠体内药动学过程属于一级吸收二室模型。
Objective: To determine bergapten's concentration in plasma and observe its pharmacokinetics in rats using a combined LC-MS/MS analytical method.Method: Blood samples were separated on a Hypersil ODS column(4.6 mm×250 mm,5 mm) at a temperature of 30 ℃,and mobile phase consisted of water and methanol(22.5∶77.5) at a flow rate of 0.8 mL·min-1.Result: The methodological study showed a good linear relationship of 8.12-162.4 g ·L-1(r=0.999 9).The inner and inter-days relative standard deviations were both less than 10%,indicating legitimate precise and accuracy to the requirement of biological sample analysis.Conclusion: The method is suitable for in vivo quantitative analysis for bergapten due to its accuracy,sensitivity and specificity.The pharmacokinetic process in rats forms a two-compartment model with first-order absorption.

关键词(KeyWords): LC-MS/MS;香柑内酯;药代动力学
LC-MS/MS;bergapten;phamacokinetics

Abstract:

Keywords:

基金项目(Foundation): 甘肃省科技计划项目﹙1011WCGA162﹚;; 兰州大学中央高校基本科研业务费专项资金项目(lzujbky-2010-191),兰州大学国家大学生创新性实验计划项目(101073039);; 教育部“春晖计划”合作科研项目(Z2009-1-62002);;

作者(Author): 马玉凤;李文;胡芳弟;石义凯;毕映燕;封士兰;
MA Yufeng1,2,LI Wen1,HU Fangdi1,SHI Yikai1,BI Yingyan1,FENG Shilan1,3(1.School of Pharmacy,Lanzhou University,Lanzhou 730000,China; 2.Hanzhong Institute of Food and Drug Control,Hanzhong 723000,China; 3.Key Laboratory of Preclinical Study for New Drugs of Gansu Province,Lanzhou 730000,China)

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