中国中药杂志

2018, v.43(04) 659-664

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人工牛黄多组分测定及其质量差异标志物的研究
Multi-component quantitation of artificial cow-bezoar and study on its markers of quality difference

石岩;孙冬梅;熊婧;魏锋;马双成;
SHI Yan;SUN Dong-mei;XIONG Jing;WEI Feng;MA Shuang-cheng;National Institutes for Food and Drug Control;Jilin Institute for Food and Drug Control;

摘要(Abstract):

建立测定人工牛黄中甘氨胆酸钠(GCANa)、胆酸(CA)、猪去氧胆酸(HDCA)、鹅去氧胆酸(CDCA)和去氧胆酸(DCA)含量的方法,并运用化学计量学方法寻找能够表征其来源的质量标志物。使用Wondasil C18(4.6 mm×250 mm,5μm)作为色谱柱;柱温25℃;以0.2%甲酸水溶液-乙腈为流动相,进行梯度洗脱,流速1.0 m L·min-1,进样量10μL,ELSD氮气流速2.3 L·min-1,漂移管温度110℃。通过化学计量学方法对样品的测定数据进行处理和分析,确定不同来源人工牛黄的质量差异标志物。方法准确可靠、简便易操作,结合质量差异标志物可适用于人工牛黄质量评价与分析。
An HPLC-ELSD method for quantitation of GCANa,CA,HDCA,CDCA and DCA in artificial cow-bezoar was developed in this study. The chromatographic separation was performed using a Wondasil C18(4. 6 mm × 250 mm,5 μm) column at a column temperature of 25 ℃ and liquid flow-rate of 1. 0 m L·min-1. Acetonitrile and 0. 2% formic acid solution were used as mobile phase with a linear gradient and injection volume was 10 μL. An ELSD was used with a nitrogen flow-rate of 2. 3 L·h-1 at a drift tube temperature of 110 ℃. Some chemometric methods were applied in data procession and analysis for exploration of the markers of quality difference.The accurate and simple method is suitable for the quality evaluation and the quality control of artificial cow-bezoar.

关键词(KeyWords): 人工牛黄;高效液相色谱法;蒸发光散射检测器;胆汁酸;化学计量学;质量标志物
artificial cow-bezoar;HPLC;ELSD;bile acids;chemometrics;quality marker

Abstract:

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作者(Author): 石岩;孙冬梅;熊婧;魏锋;马双成;
SHI Yan;SUN Dong-mei;XIONG Jing;WEI Feng;MA Shuang-cheng;National Institutes for Food and Drug Control;Jilin Institute for Food and Drug Control;

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