中国中药杂志

2011, v.36(06) 725-728

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千里光与欧洲千里光非生物碱部位HPLC特征指纹图谱比较研究
Study on HPLC fingerprints of Senecio scandens and S.scandens

杨雪晶;熊爱珍;杨莉;王长虹;王峥涛;
YANG Xuejing1,XIONG Aizhen2,YANG Li2,WANG Changhong2,WANG Zhengtao1,2,3* (1.Department of Pharmacognosy,China Pharmaceutical University,Nanjing 210038,China; 2.The MOE Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 3.Shanghai Key Laboratory for Compound Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,Chin

摘要(Abstract):

目的:建立千里光Senecio scandens和欧洲千里光S.scandens非生物碱部位的HPLC特征指纹图谱分析方法,并对二者进行比较研究。方法:采用HPLC-UV方法对7批千里光药材和6批欧洲千里光药材的指纹图谱进行了比较,并分别以绿原酸、金丝桃苷为对照品,对指纹峰进行了半定量分析;采用Shiseido Capcell Pak MGⅡC18(4.6 mm×250 mm,5μm)色谱柱,流动相乙腈-0.2%乙酸,梯度洗脱。结果:2种千里光属植物HPLC非生物碱部位的特征指纹图谱差异显著,分别在千里光和欧洲千里光指纹谱中检出11个共有峰,HPLC-DAD及HPLC-MS分析结果表明上述共有峰主要为有机酸类(包括绿原酸类和原儿茶酸类化合物)和黄酮类化合物。半定量分析的结果表明千里光中有机酸和黄酮类化合物的含量较高,其平均含量分别为欧洲千里光中的2.39,15.56倍。结论:所建立HPLC方法稳定可靠,同时适用于千里光属植物千里光和欧洲千里光的指纹图谱研究,可用于千里光药材的质量评价。
Objective: To develop an HPLC method for fingerprint study of both Senecio scandens and S.scandens.Method: Fingerprints of the two Senecio herbs were compared.And the concentrations of main peaks in them were semi-quantified as chlorogenic acid or hyperoside.Chromatography was performed on a Shiseido Capcell Pak MG Ⅱ C18(4.6 mm×250 mm,5 μm) column using a gradient elution of acetonitrile-water containing 0.2% acetic acid.And detection wavelength was 360 nm.Result: Significant difference was found in the fingerprint of the two herbs.Eleven peaks were picked out for the evaluation of S.scandens and S.scandens,respectively.They were identified to be either organic acid compounds or flavones by HPLC-UV and LC-ESI-MS analysis.And semi-quantification of them showed the concentrations of organic acid compounds and flavones in S.scandens were 2.29-and 15.56-folds of those in S.scandens,respectively.Conclusion: The developed HPLC method is suitable for the fingerprint study for both of S.scandens and S.scandens.It is robust and producible enough to be used for the quality evaluation on S.scandens.

关键词(KeyWords): 千里光;欧洲千里光;有机酸;黄酮;指纹图谱
Senecio scandens;S.vulgaris;organic acid compounds;flavones;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09308-003,2009ZX09052-013,2009ZX09052-021,2009ZX09052-024);; 国家自然科学基金项目(30701104);; 上海市国际合作基金项目(09540704700);; 中医药行业科研专项项目(200707009)

作者(Author): 杨雪晶;熊爱珍;杨莉;王长虹;王峥涛;
YANG Xuejing1,XIONG Aizhen2,YANG Li2,WANG Changhong2,WANG Zhengtao1,2,3* (1.Department of Pharmacognosy,China Pharmaceutical University,Nanjing 210038,China; 2.The MOE Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 3.Shanghai Key Laboratory for Compound Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,Chin

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