林生,张启伟,张宁宁,张永欣
LIN Sheng, ZHANG Qi-wei, ZHANG Ning-ning, ZHANG Yong-xin(Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China)

• 1:中国中医研究院中药研究所
• 2:中国中医研究院中药研究所
• 3:中国中医研究院中药研究所
• 4:中国中医研究院中药研究所 北京100700

摘要(Abstract)：

目的:建立花蕾期茵陈4个黄酮类成分的含量测定方法。方法:样品用醋酸乙酯超声提取30min。KromasilODS柱,流动相乙腈四氢呋喃 1%醋酸水溶液(2 5∶2 1. 5∶76 ) ,检测波长347nm。结果:茵陈花蕾中的4个黄酮类成分5 ,7,4′. 三羟基 6 ,2′,5′. 三甲氧基黄酮(arcapillin) ,5 ,3′,4′. 三羟基 6 ,7 .二甲氧基黄酮(cirsiliol) ,蓟黄素(cirsimaritin)和3′甲氧基蓟黄素(cirsilineol)在该色谱条件下获良好分离,分别在0 . 0 35～0 5 6 ,0 . 0 4 3～0 . 6 9,0 . 0 4 0～0. 6 5 ,0 . 0 4 0～0 6 4 μg线性关系良好(r =0 . 9992 ,0 9999,0 . 9997,0 9997) ,加样回收率分别为96. 8% ,97. 3% ,96. 8% ,98 .5 % ,重复性分别为2 . 5 % ,3 0 % ,1 5 % ,3. 2 %。结论:该方法分离度好,为花蕾期茵陈及其制剂的质量控制提供。
Objective: To develop a quantitative method for the determination of four flavonoids in buds of Herba Artemisiae Scopariae. Method: The sample was extracted by ultrasonic with ethyl acetate for 30 minutes and separated on kromasil ODS column with mobile phase of acetonitrile-tetrahydrofuran-1% acetic acid solution(2.5∶21.5∶76)and detection wavelength was 347 nm. Result: The arcapillin, cirsiliol, cirsimaritin and cirsilineol in bud extract were well separated by this method. Linearities of arcapillin, cirsiliol, cirsimaritin and cirsilineol were good(r=0.999 2,0.999 9,0.999 7,0.999 7)in ranges of 0.035-0.56,0.043-0.69,0.040-0.64 and 0.040-0.64 μg,respectively. The average recoveries were 96.8 %,97.3%,96.8%,98.5% and RSD the values of repeatability were 2.5%,3.0%,1.5%,3.2%, respectively. Conclusion: The method validation data indicated that the method is reliable and it can be used for quality control of Herba Artemisiae Scopariae and its preparations.

关键词(KeyWords)： 花蕾期茵陈;黄酮;高效液相色谱法
Herba Artemisiae Scopariae collected in autumn; flavonoids; HPLC

Abstract:

Keywords:

基金项目(Foundation): 国家重点科技攻关项目 (96 90 3 0 2 0 2 )

作者(Author): 林生,张启伟,张宁宁,张永欣
LIN Sheng, ZHANG Qi-wei, ZHANG Ning-ning, ZHANG Yong-xin(Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China)

Email:

DOI:

参考文献(References)：

• [1]　张启伟,章育中滨蒿中利胆成分的含量测定药学学报,1986,21(12):922
• [2]　张启伟,章育中滨蒿中利胆成分的薄层光密度法测定药学学报,1989,24(1):43
• [3]　王喜军,孙　晖,刘中申茵陈蒿及其复方制剂中6,7-二甲氧基香豆素和茵陈色原酮的定量研究中国中药杂志,1994,19(11):667
• [4]　SheuSJ,TanYW DeterminationofphenoliccompoundsinArtemisi acapillarisJHighResolutChromatogr,1999,22(4):222
• [5]　SheuSJ,ChiehCL ,WengWC Capillaryelectrophoreticdetermina tionoftheconstitunentsofArtemisiaeCapollarisHerbaJChromatogrA ,2001,911(2):285
• [6]　张启伟,张永欣,张　颖,等高效液相色谱法测定花蕾期茵陈中3′甲氧基蓟黄素的含量中国中药杂志,2002,27(1):23
• [7]　KisoY ,OgasawaraS ,HirotaK ,etalAntihepatoxicprinciplesofArtemisiacapillarisbudsPlantaMed,1984,46(1):81
• [8]　蒋洁云,徐　强,王　蓉,等茵陈抗肿瘤活性成分的研究中国药科大学学报,1992,23(5):283
• [9]　LIUYL ,HoDK ,CassadyJM Isolationofpotentialcancerchemo preventiveagentsfromeridictyoncalifornicumNatProd,1992,55(3):357
• [10]　陈惠芳植物活性成分辞典第二册北京:中国医药科技出版社,2001102
• [11]　sugiyamaS ,UmenaraK ,KuroyanayiM ,etalStudiesonthediffer entinationinducersofmyeloidleukemiaccellsfromcitrusspeciesChemPhamBull,1992,41(4):714
• [12]　NikaidoT ,QumotoT ,SankawaV ,etalInhibitionofadenosine3,5 cylicmonophosphatephosphodiesterasebyflavonoidsⅡChemPhamBull,1988,36(2):654
• [13]　张启伟,张永欣,张　颖,等滨蒿化学成分的研究中国中药杂志,2002,27(3):202
• [14]　CasteeleKV ,GeigerH ,VanSumereCF Seperationofflavonoidsbyreversed phasehigh performanceliquidchromatographyJChro matogr,1982,240(1):81
• [15]　NyiredySz,MeierB ,ErdelmeierCAJ ,etal“Prisma”:ageomet ricaldesignforsolventoptimizationinHPLC JHighResolutChro matogrChromatogrCommun,1985,8(4):186