王筱亮,张玉杰,陈明霞,王泽凤
WANG Xiao-liang, ZHANG Yu-jie, CHEN Ming-xia, WANG Ze-feng(Department of Chinese Pharmacy , Beijing University of Traditional Chinese Medicine, Beijing 100102, China)

• 1:北京中医药大学中药学院
• 2:北京中医药大学中药学院
• 3:北京中医药大学中药学院
• 4:北京中医药大学中药学院 北京100102

摘要(Abstract)：

目的:分别以酸枣仁中总皂苷量、酸枣仁皂苷A,B总量、总黄酮量为检测指标对酸枣仁进行提取工艺优选。方法:采用单因素考察和正交试验相结合的方法,正交试验中以乙醇含量(A)、提取次数(B)、提取时间(C)、溶剂用量(D)4个因素,每个因素选取3个水平进行试验。结果:因素A,B对检测指标均有显著性影响,因素C,D为不显著因素,可结合生产实际进行选择。结论:以60%乙醇,8倍量提取3次,每次1.5h为最佳提取工艺。
Objective: To optimize extraction technology of the seed of Ziziphus jujuba var. spinosa with the targets of the total saponin, total jujuboside A and B and total flavonoids. Method: In the method of one-way and orthogonal tests, ethanol concentration, amount of ethanol, extraction time and extraction times were the factors in orthogonal test, and each factor with three levels. Result: Ethanol concentration and extraction times had significant effect on all the targets, other factors should be selected in accordance with production practice. Conclusion: The best extraction technology is to extract for three times with 8 fold ethanol solution(60%), and 1.5 h each time.

关键词(KeyWords)： 酸枣仁;总皂苷;酸枣仁皂苷A,B;总黄酮;正交试验;提取工艺
seed of Ziziphus jujuba var.spinosa; multitarget; saponins; jujubosidesA and B; flavonoids

Abstract:

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基金项目(Foundation):

作者(Author): 王筱亮,张玉杰,陈明霞,王泽凤
WANG Xiao-liang, ZHANG Yu-jie, CHEN Ming-xia, WANG Ze-feng(Department of Chinese Pharmacy , Beijing University of Traditional Chinese Medicine, Beijing 100102, China)

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参考文献(References)：

• [1]中国药典.一部.2000.300.
• [2]王建,林晓.酸枣仁中酸枣仁皂苷A和B的含量测定.中草药,1996,27(3):154.
• [3]丁慎德.枣仁有效成分的研究.淄博师专学报,1995,(4):56.