中国中药杂志

2017, v.42(11) 2046-2051

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药用植物DNA标记辅助育种(三)三七新品种——“苗乡抗七1号”的抗病性评价
DNA marker-assisted selection of medicinal plants(Ⅲ) Evaluation of disease resistance of “Miaoxiang Kangqi 1”——a new cultivar of Panax notoginseng

陈中坚;马小涵;董林林;张连娟;尉广飞;肖丽娜;王勇;魏福刚;刘伟林;余育启;陈士林;
CHEN Zhong-jian;MA Xiao-han;DONG Lin-lin;ZHANG Lian-juan;WEI Guang-fei;XIAO Li-na;WANG Yong;WEI Fu-gang;LIU Wei-lin;YU Yu-qi;CHEN Shi-lin;Institute of Sanqi Research,Wenshan University;Institute of Chinese Materia Media,China Academy of Chinese Medicinal Sciences;Wenshan Miaoxiang Notoginseng Technology Co.,Ltd.;

摘要(Abstract):

药用植物DNA标记辅助育种加快新品种选育及推广的进程,保障中药材产业健康发展。该研究以首个三七DNA标记辅助选育新品种—"苗乡抗七1号"为研究对象,系统评价其种子、种苗、块根对根腐病致病菌Fusarum oxysporum的抗性。结果表明,与常规栽培种相比,接种7 d,抗病品种种子病情指数下降52.0%;接种25 d,抗病品种种苗死苗率及块根病情指数分别下降72.1%,62.4%;此外,接种后抗病品种种子及种苗生长抑制率下降。"苗乡抗七1号"种子、种苗、块根对根腐病表现显著的抗性,该品种的抗性评价将为新品种的推广提供依据,保障三七无公害栽培的顺利开展。
DNA marked-assisted selection of medicinal plants accelerated the breeding and promotion of new cultivars,and guaranteed the healthy development of Chinese medicinal materials industry. The first disease-resistant cultivar of notoginseng,namely " Miaoxiang Kangqi 1",served as the object of study. We evaluated the Kangqi's resistance of seeds,seedlings and root against the pathological bacteria( Fusarum oxysporum) of root rot. Compared to the traditional cultivars,the disease index of notoginseng seeds declined by 52. 0% after inoculation for seven days; the death rate of seedlings and disease index of root respectively decreased by 72. 1% and62. 4% after inoculation for 25 days. Additionally,the growth inhibition ratio of notoginseng seeds and seedlings declined after inoculation.The seeds,seedlings and roots of"Miaoxiang Kangqi 1"showed significantly resistant to root rot. The evaluation of disease-resistance of Kangqi provided the basis for the popularization of new cultivar and guaranteed the favoring conduct of notoginseng pollution-free cultivation.

关键词(KeyWords): 三七;根腐病;抗病品种;发病率;病情指数
Panax notoginseng;root rot;disease-resistant cultivars;incidence rate;disease index

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81603238)

作者(Author): 陈中坚;马小涵;董林林;张连娟;尉广飞;肖丽娜;王勇;魏福刚;刘伟林;余育启;陈士林;
CHEN Zhong-jian;MA Xiao-han;DONG Lin-lin;ZHANG Lian-juan;WEI Guang-fei;XIAO Li-na;WANG Yong;WEI Fu-gang;LIU Wei-lin;YU Yu-qi;CHEN Shi-lin;Institute of Sanqi Research,Wenshan University;Institute of Chinese Materia Media,China Academy of Chinese Medicinal Sciences;Wenshan Miaoxiang Notoginseng Technology Co.,Ltd.;

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DOI: 10.19540/j.cnki.cjcmm.20170519.001

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