中国中药杂志

2015, v.40(04) 716-721

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麦冬多糖MDG-1对膳食诱导肥胖模型小鼠肠道益生菌群多样性影响的研究
Effect of MDG-1,a polysaccharide from Ophiopogon japonicas,on diversity of lactobacillus in diet-induced obese mice

石林林;王源;冯怡;
SHI Lin-lin;WANG Yuan;FENG Yi;Shanghai University of Traditional Chinese Medicine,Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine of Ministry of Education;

摘要(Abstract):

目的:研究麦冬多糖MDG-1对膳食诱导肥胖小鼠肠道益生菌群多样性的影响,特别关注肠道益生乳酸菌多样性的变化。方法:选取8周龄雄性C57BL/6J小鼠60只,给予高脂饲料(research diets,D12492)喂养6周及适应性灌胃2周,剔除体重低于30 g和灌胃体重降幅超过10%的动物,将诱导成功的肥胖模型小鼠按体重和血糖随机分为DIO模型组(diet-induced obesity,DIO)以及MDG-1高(300 mg·kg-1)、中(150 mg·kg-1)、低(75 mg·kg-1)剂量给药组;模型组及MDG-1不同浓度治疗组用高脂饲料喂养,对照组小鼠始终给予低脂饲料(research diets,D12450B)喂养,每组各12只。治疗组小鼠分别以不同浓度的MDG-1溶液灌胃,模型组和对照组小鼠以对照溶剂(生理盐水)灌胃,持续16周,于治疗终点收集各组小鼠直肠内容物。通过传统培养方法结合变性梯度凝胶电泳(DGGE)法对小鼠的肠道益生菌进行培养,统计菌落数量,分析模型动物口服不同浓度的MDG-1前后肠道益生菌群的变化,通过16S r DNA测序,建立系统发育树以及利用PCR-DGGE技术等分子鉴定手段确定小鼠肠道菌群变化的优势菌群。结果:不同浓度的MDG-1均可增加小鼠肠道益生菌群数量,尤其是鼠乳杆菌和台湾乳杆菌,且随浓度增加作用逐渐增强。结论:不同给药剂量的MDG-1可在一定程度上增加小鼠肠道益生菌的数量,尤其是台湾乳杆菌和鼠乳杆菌,同时改善肠道菌群多样性,促进肠道益生菌的增殖。
For understanding the effect of MDG-1,a water-soluble β-D-fructan polysaccharide from Ophiopogon japonicas,on intestinal microecological balance,especially on the changes of lactobacillus,sixty 8-week-old male C57 BL /6J mice were given a high-fat diet for six weeks and were also gavaged with saline once a day simultaneously. Then the mice which is below 30 grams or dropped more than 10% through lavage were eliminated and the rest were randomly divided into four groups: diet-induced obese( DIO) model group( n = 12,gavaged with saline),low-dose MDG-1 group( n = 12,gavaged with MDG-1,75 mg · kg- 1),medial-dose MDG-1group( n = 12,gavaged with 150 mg·kg- 1),and high-dose MDG-1group( n = 12,gavaged with 300 mg·kg- 1) according to the weight and blood glucose; the model group and MDG-1 group were placed on a high-fat diet while the normal control group( n = 12,gavaged with saline) were kept on a low-fat diet through the experiment. After 12-weeks of treatment,feces samples were collected and cultured for intestinal microecological balance analysis. Then the intestinal probiotics were cultured through traditional methods combined with modified gradient gel electrophoresis( DGGE) method. The changes of lactobacillus in each treatment group were also detected by a statistical analysis of the total number of the intestinal flora. We have established the phylogenetic tree by 16 S r DNA sequencing and use some molecular identification methods such as PCR-DGGE to analyse the changes of the dominant bacteria floras,and also get the pure culture. In conclusion,different concentrations of MDG-1can increase the number of the intestinal probiotics,especially Taiwan lactobacillus and Lactobacillus murinus,and improve their diversity and promote proliferation in a dose-dependent way.

关键词(KeyWords): 麦冬多糖;MDG-1;肥胖;肠道菌群;多样性
MDG-1;obesity;intestinal flora;diversity

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81173516);; 上海市教委一流学科(ZYX-CXYJ-016);; 高校博士点基金新教师类(20133107120004);高校博士点基金博导类(20133107110005)

作者(Author): 石林林;王源;冯怡;
SHI Lin-lin;WANG Yuan;FENG Yi;Shanghai University of Traditional Chinese Medicine,Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine of Ministry of Education;

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