中国中药杂志

2020, v.45(15) 3726-3739

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

清肺排毒汤对大鼠整体代谢及肠道菌群的调节作用研究
Investigation of modulating effect of Qingfei Paidu Decoction on host metabolism and gut microbiome in rats

吴高松;钟婧;郑宁宁;王超然;金红利;葛广波;韩晶岩;高月;盛丽莉;张卫东;李后开;
WU Gao-song;ZHONG Jing;ZHENG Ning-ning;WANG Chao-ran;JIN Hong-li;GE Guang-bo;HAN Jing-yan;GAO Yue;SHENG Li-li;ZHANG Wei-dong;LI Hou-kai;Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine;Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Affiliated Central Hospital of Huzhou University;Dalian Institute of Chemical Physics, Chinese Academy of Sciences;Innovation Institute of Medicine, China Medical City, Dalian Inst

摘要(Abstract):

通过代谢组学和肠道微生物组学探讨清肺排毒汤(Qingfei Paidu Decoction,QPD)对大鼠整体代谢及肠道菌群的影响,为揭示清肺排毒汤的临床药效机制提供实验依据。采用GC-MS结合LC-MS/MS的非靶向代谢组学以及肠道微生物组学方法,对清肺排毒汤干预大鼠5 d后的血清内源性代谢物谱与肠道菌群组成开展了系统的研究。基于GC-MS和LC-MS/MS代谢组学方法分别获得清肺排毒汤调控的23种和43种差异代谢物,涉及的代谢通路主要包括甘油磷脂代谢、亚油酸代谢、TCA循环和丙酮酸代谢。利用基于16S rDNA测序分析发现清肺排毒汤显著调节了大鼠肠道菌群的组成,明显上调Romboutsia,Turicibacter,Clostridium_sensu_stricto_1的相对丰度,下调norank_f_Lachnospiraceae的相对丰度。该研究证明清肺排毒汤短期干预能够显著调节大鼠整体代谢,并调节动物的肠道菌群组成,提示清肺排毒汤的临床疗效可能与其对机体的内源性代谢调控及肠道菌群组成的调节有关。
This study is to explore the effect of Qingfei Paidu Decoction(QPD) on the host metabolism and gut microbiome of rats with metabolomics and 16 S rDNA sequencing. Based on 16 S rDNA sequencing of gut microbiome and metabolomics(GC-MS and LC-MS/MS), we systematically studied the serum metabolites profile and gut microbiota composition of rats treated with QPD for continued 5 days by oral gavage. A total of 23 and 43 differential metabolites were identified based on QPD with GC-MS and LC-MS/MS, respectively. The involved metabolic pathways of these differential metabolites included glycerophospholipid metabolism, linoleic acid metabolism, TCA cycle and pyruvate metabolism. Meanwhile, we found that QPD significantly regulated the composition of gut microbiota in rats, such as enriched Romboutsia, Turicibacter, and Clostridium_sensu_stricto_1, and decreased norank_f_Lachnospiraceae. Our current study indicated that short-term intervention of QPD could significantly regulate the host metabolism and gut microbiota composition of rats dose-dependently, suggesting that the clinical efficacy of QPD may be related with the regulation on host metabolism and gut microbiome.

关键词(KeyWords): 清肺排毒汤;新型冠状病毒肺炎;代谢组学;肠道微生物组学;炎症;免疫
Qingfei Paidu Decoction;COVID-19;metabolomics;gut microbiome;inflammation;immune

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2020YFC0845400,2017YFC1700200)

作者(Author): 吴高松;钟婧;郑宁宁;王超然;金红利;葛广波;韩晶岩;高月;盛丽莉;张卫东;李后开;
WU Gao-song;ZHONG Jing;ZHENG Ning-ning;WANG Chao-ran;JIN Hong-li;GE Guang-bo;HAN Jing-yan;GAO Yue;SHENG Li-li;ZHANG Wei-dong;LI Hou-kai;Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine;Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Affiliated Central Hospital of Huzhou University;Dalian Institute of Chemical Physics, Chinese Academy of Sciences;Innovation Institute of Medicine, China Medical City, Dalian Inst

Email:

DOI: 10.19540/j.cnki.cjcmm.20200609.201

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享