中国中药杂志

2018, v.43(14) 2878-2883

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滋肾育胎丸指纹图谱及其5种指标成分含量测定的研究
HPLC fingerprint of Zishen Yutai pills and simultaneous determination of 5 index components

马蕙文;邹琦;李春;尹震;易红;田莲超;任承涛;刘晓谦;王智民;
MA Hui-wen;ZOU Qi;LI Chun;YIN Zhen;YI Hong;TIAN Lian-chao;REN Cheng-tao;LIU Xiao-qian;WANG Zhi-min;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University;Guangzhou Baiyunshan Zhongyi Pharmaceutical Co.,Ltd.;

摘要(Abstract):

采用高效液相色谱法分别建立了滋肾育胎丸指纹图谱及马钱苷酸、绿原酸、马钱苷、当药苷和川续断皂苷Ⅵ5个成分同时测定的方法,以期对其质量控制提供科学依据。采用中药色谱指纹图谱相似度评价系统(2012)对11批滋肾育胎丸样品进行分析,建立滋肾育胎丸指纹图谱,在254 nm下标定了15个共有峰,指纹图谱相似度均>0.90。所建立的5个指标成分同步测定的方法,各成分在各自质量浓度范围内与峰面积呈良好线性关系(r≥0.999 2),平均回收率在97.62%~101.9%,RSD均<3.0%。该研究所建立的指纹图谱和定量分析的方法简便、准确度高,能更全面反映滋肾育胎丸的质量,可用于滋肾育胎丸的质量控制。
To establish the HPLC fingerprint and determine five index components(loganic acid,chlorogenic acid,loganin,sweroside and asperosaponin Ⅵ) of Zishen Yutai pills by high performance liquid chromatography,and provide a scientific basis for its quality control. The fingerprint chromatogram was analysed by the chromatographic fingerprint similarity evaluation system for tradition Chinese medicine(2012),fifteen common peaks were obtained at the wavelength of 254 nm. Different batches of Zishen Yutai pills showed a similarity of above 0. 90 in HPLC fingerprint profiles. For the quantitive analysis method,The separation of five components showed good regression(r≥0. 999 2) with linear ranges,and the mean recoveries were in the range of 97. 62%-101. 9%,with the RSD(n = 9) less than 3%. The established fingerprint and quantitative analysis methods are highly specific,simple and accurate,which can reflect the quality of Zishen Yutai pills more comprehensively,and can be used for its quality control.

关键词(KeyWords): 滋肾育胎丸;指纹图谱;马钱苷酸;绿原酸;马钱苷;当药苷;川续断皂苷Ⅵ
Zishen Yutai pills;fingerprint;loganic acid;chlorogenic acid;loganin;sweroside;asperosaponin Ⅵ

Abstract:

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基金项目(Foundation): 国家中药标准化项目(ZYBZH-C-GD-07);; 广东省中医药局科技项目

作者(Author): 马蕙文;邹琦;李春;尹震;易红;田莲超;任承涛;刘晓谦;王智民;
MA Hui-wen;ZOU Qi;LI Chun;YIN Zhen;YI Hong;TIAN Lian-chao;REN Cheng-tao;LIU Xiao-qian;WANG Zhi-min;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University;Guangzhou Baiyunshan Zhongyi Pharmaceutical Co.,Ltd.;

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