中国中药杂志

2019, v.44(21) 4573-4580

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干旱胁迫对荆芥腺毛与气孔密度及腺毛分泌物的影响
Effects of drought stress on glandular trichomes,stomatal density and volatile exudates of Schizonepeta tenuifolia

李柯;李四菊;周庄煜;姚浩铮;周莹;唐晓清;王康才;
LI Ke;LI Si-ju;ZHOU Zhuang-yu;YAO Hao-zheng;ZHOU Ying;TANG Xiao-qing;WANG Kang-cai;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;

摘要(Abstract):

该研究采用盆栽控水试验,探究不同水分处理[栽培基质相对含水量分别为5%~10%(重度干旱胁迫),45%~50%(中度干旱胁迫),85%~90%(对照组)]和干旱胁迫时间(胁迫15,30,45 d)对荆芥腺毛密度、气孔密度以及腺毛分泌物的影响。结果表明,荆芥叶表面存在2种腺毛:头状腺毛和盾状腺毛。叶片上下表皮的头状腺毛密度均高于盾状腺毛,且叶下表皮两种腺毛密度都高于上表皮。在重度干旱胁迫下,荆芥叶表皮的头状腺毛密度、气孔密度有所增加。在同一胁迫时间下,荆芥的生物量和叶表面积随胁迫程度的加深而降低,随着胁迫时间延长,生物量和叶表面积逐渐增大,腺毛和气孔密度降低。胡薄荷酮是荆芥腺毛分泌物中相对含量最高的成分。在干旱胁迫下,胡薄荷酮的相对含量下降,柠檬烯、薄荷酮等其他单萜类成分相对含量升高。棕榈酸和2甲基-1-十六醇仅在胁迫15 d时的荆芥分泌物中检出,而薄荷酮在胁迫30 d和45 d时被检出。干旱胁迫影响荆芥的叶片生长和次生代谢,该研究可为干旱条件下荆芥的水分管理及进一步品质调控提供理论参考。
In this research,we explored the effect of three groups of water treatments,including severe drought(the corresponding water content of cultivated substrate 5%-10%),moderate drought(45%-50%) and control(85%-90%),and different drought stress time(15,30,45 d) on the glandular trichome density(TD),stomatal density(SD) and volatile exudates of Schizonepeta tenuifolia.The results showed that there were two kinds of glandular trichomes on the surface of S. tenuifolia leaves: peltate and capitate glandular trichomes. The density of capitate glandular trichomes(CTD) was higher than that of peltate glandular trichomes(PTD). Both CTD and PTD on the abaxial surface of leaf were higher than those on the adaxial surface. Under severe drought stress,the CTD and SD were higher than the other two treatments. Under the same stress time,the biomass and leaf surface area of S. tenuifolia decreased with the deepening of stress degree. As the stress time prolonged,the surface area of leaves and biomass gradually increased,and the TD and SD decreased. The most abundant compound in volatile exudates of S. tenuifolia was pulegone. Under drought stress,the relative content of pulegone decreased,and the relative content of other monoterpenoids such as D-limonene and menthone increased. The n-hexadeconic acid and 2-methyl-1-hexadecanol were detected only at the stress of 15 d,while menthone was detected at the stress of 30 d and45 d. Drought stress affected the leaf growth and secondary metabolism of S. tenuifolia.

关键词(KeyWords): 干旱胁迫;荆芥;腺毛密度;气孔密度;腺毛分泌物
drought stress;Schizonepeta tenuifolia;glandular trichomes density;stomatal density;volatile exudates

Abstract:

Keywords:

基金项目(Foundation): 中央高校基本科研业务费项目(KYZZ201913);; 2018年中医药公共卫生服务补助专项(财社[2018]43号);; 南京农业大学大学生创新创业训练计划项目(2018syzx010)

作者(Author): 李柯;李四菊;周庄煜;姚浩铮;周莹;唐晓清;王康才;
LI Ke;LI Si-ju;ZHOU Zhuang-yu;YAO Hao-zheng;ZHOU Ying;TANG Xiao-qing;WANG Kang-cai;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20190829.104

参考文献(References):

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