中国中药杂志

2017, v.42(04) 657-662

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广藿香内生真菌类群分析及其抗菌活性研究
Study on communities of endophytic fungi from Pogostemon cablin and their antimicrobial activities

王营;李浩华;谭国慧;李赛妮;严明理;章卫民;
WANG Ying;LI Hao-hua;TAN Guo-hui;LI Sai-ni;YAN Ming-li;ZHANG Wei-min;School of Chemistry and Chemical Engineering,Hunan University of Science and Technology;State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangdong Institute of Microbiology;

摘要(Abstract):

从广藿香的根、茎、叶中共分离得到72株内生真菌,根据ITS序列分析鉴定为25属40个种,其中拟茎点霉属Phomopsis、刺盘孢属Colletotrichum、镰刀菌属Fusarium为优势种群;广藿香内生真菌的分布存在明显的组织特异性,以茎中内生真菌的分布最多,占分离菌株总数的78%;活性测试结果显示共有15个属34株内生真菌至少对1种供试菌具有抗菌活性。结果表明广藿香内生真菌多样性丰富,部分菌株具有明显的抑菌活性,值得进一步深入研究。
Seventy-two strains of endophytic fungi were isolated from roots,stems and leaves of Pogostemon cablin and identified as40 species of 25 genera based on ITS sequences analysis. Among them,Phomopsis,Colletotrichum and Fusarium were dominant genera. Distribution of endophytic fungi in P. cablin showed obvious tissue-specificity,and more strains were isolated from stems with an isolation rate of 78%. The bioassay results indicated that 34 strains of 15 genera displayed antimicrobial activities against at least one of test bacteria or plant pathogenic fungi. The results obtained in this study showed that endophytic fungi in P. cablin were rich in species diversity,and some strains exhibited strong antimicrobial activities,which deserve further research.

关键词(KeyWords): 内生真菌;多样性;广藿香;抗菌活性
endophytic fungi;diversity;Pogostemon cablin;antimicrobial activity

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基金项目(Foundation): 国家重点基础研究发展计划(973)前期专项(2014CB460613);; 广东省自然科学基金项目(2015A030313710);; 广东省科技计划项目(2015A030302060,2014A030304050)

作者(Author): 王营;李浩华;谭国慧;李赛妮;严明理;章卫民;
WANG Ying;LI Hao-hua;TAN Guo-hui;LI Sai-ni;YAN Ming-li;ZHANG Wei-min;School of Chemistry and Chemical Engineering,Hunan University of Science and Technology;State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangdong Institute of Microbiology;

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