中国中药杂志

2020, v.45(14) 3414-3421

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生防菌BZJN1和苯甲·丙环唑对白术根际土壤细菌群落组成及理化特性的影响
Effects of biocontrol strain BZJN1 and streptoprofen on physicochemical properties and bacteria structure of rhizosphere soil of Atractylodes macrocephala

唐涛;毛婷;郭杰;王帆帆;方国斌;林先明;匡辉;孙光忠;段媛媛;郭晓亮;游景茂;
TANG Tao;MAO Ting;GUO Jie;WANG Fan-fan;FANG Guo-bin;LIN Xian-ming;KUANG Hui;SUN Guang-zhong;DUAN Yuan-yuan;GOU Xiao-liang;YOU Jing-mao;Institute of Chinese Herbal Medicines, Hubei Academy of Agriculture Science;Hubei Minzu University;the Vegetational Protection Union Station of Hubei Province;

摘要(Abstract):

土壤微生物是重要的土壤质量生物学指标之一,土壤微生物数量能够一定程度上反映土壤的综合生态环境特征,对土壤微生物多样性的研究是研究土壤生态功能和土壤生态平衡的关键。该文基于细菌16S rRNA V3~V4区高通量测序技术,分析比较化学药剂苯甲·丙环唑处理组和生防菌BZJN1处理组与对照组白术根际土壤中细菌多样性和群落结构,采用冗余分析(redundancy analysis,RDA)探讨土壤环境因子与细菌多样性的联系。结果表明:化学药剂苯甲·丙环唑处理组和生防菌BZJN1处理组与对照组白术根际土壤中细菌无显著差异,各土壤样品中共检测到细菌25门67纲108目167科271属,主要的优势菌门为变形菌门(Proteobacteria 30.7%~34.8%),其中α-变形菌纲(Alphaproteobacteria,16.8%~18.5%)为优势亚群。相较于对照组,化学药剂苯甲·丙环唑处理组和生防菌BZJN1处理组白术根际土壤多个门细菌相对丰度发生显著变化;此外,RDA分析表明,不同环境因子与微生物群落有一定联系,白术根际土壤中3个属群落丰度与Invertase、Urease和AP显著正相关。PICRUSt功能预测结果显示,BZNJ1能增强部分细菌功能促进植物生长。生防菌作为新型绿色安全控害方法,BZNJ1在有效防治白术根腐病、促进植物生长的基础上显著增强了部分细菌功能,对土壤细菌群落结构没有显著影响,该结果为生防菌BZJN1的推广运用提供理论支持。
Soil microorganisms are one of the important biological indictors of soil quality and can reflct the comprehensive ecological environment characteristics of the soil. The research of soil microbial diversity is the key to know the ecological functions and balance with soil. In this paper, high-throughput sequencing on PCR-amplified 16 S rRNA gene V3-V4 fragments was used to determine the bacterial diversity in rhizosphere soil of A. macrocephala under the treatment with BZJN1 or streptoprofen. The results showed that there were no significant differences of the bacteria in A. macrocephala rhizosphere soil of the streptoprofen treatment group and the biocontrol BZJN1 treatment group. All the soil bacteria was classified into 25 categories,67 classes, 108 orders, 167 families and 271 generas, except some unidentified bacteria. Proteobacteria(30.7%-34.8%) was the dominant phylum, of which Alphaproteobacteria(16.8%-18.5%) was the dominant subgroup. Compared with the control group, the relative abundance of multiple phylums bacteria in the rhizosphere soil of A. macrocephala was significantly changed in the streptoprofen treatment group and the biocontrol BZJN1 treatment group. In addition, RDA analysis showed that there was connection with different environmental factors and microbial communities. The abundance of the three genera in the rhizosphere soil of A. macrocephala was significantly positively correlated with Invertase, Urease and AP. PICRUSt function prediction results showed that BZNJ1 could enhance some bacterial functions and promote the plant growth. Biocontrol is a new type of green and safety control pest method. BZNJ1 significantly enhances some bacterial functions on the basis of effectively preventing root rot of A. macrocephala and promoting plant growth, and has no significant effect on the soil bacterial community structure. All the results can provide theoretical support for popularization of BZNJ1.

关键词(KeyWords): 生防菌BZJN1;苯甲·丙环唑;细菌群落组成;高通量测序
biocontrol strain BZJN1;streptoprofen;bacterial community structure;high-throughput sequencing

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系建设专项(CARS-21);; 湖北省技术创新专项(2018AKB037);; 湖北省农业科学院扶持性计划项目(2019fcxjh09);湖北省农业科学院青年基金项目(2018NKYJJ16,2020NKYJJ20)

作者(Author): 唐涛;毛婷;郭杰;王帆帆;方国斌;林先明;匡辉;孙光忠;段媛媛;郭晓亮;游景茂;
TANG Tao;MAO Ting;GUO Jie;WANG Fan-fan;FANG Guo-bin;LIN Xian-ming;KUANG Hui;SUN Guang-zhong;DUAN Yuan-yuan;GOU Xiao-liang;YOU Jing-mao;Institute of Chinese Herbal Medicines, Hubei Academy of Agriculture Science;Hubei Minzu University;the Vegetational Protection Union Station of Hubei Province;

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