中国中药杂志

2019, v.44(17) 3615-3621

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葛根SSR特征及江西粉葛DNA身份证的构建
Analysis of genomic SSRs in Pueraria lobata and P. thomsonii and establishment of DNA identity card for different germplasms of P. thomsonii of Jiangxi province

周荣荣;周骏辉;南铁贵;蒋超;段海燕;赵宇平;黄璐琦;袁媛;
ZHOU Rong-rong;ZHOU Jun-hui;NAN Tie-gui;JIANG Chao;DUAN Hai-yan;ZHAO Yu-ping;HUANG Lu-qi;YUAN Yuan;School of Pharmacy,Nanjing University of Chinese Medicine;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

使用生物信息学方法分析野葛Pueraria lobata和甘葛藤P. thomsonii基因组SSR(simple sequence repeat)序列。分别获得66 191,71 968个SSR位点,据此开发了20对SSR引物,从中筛选出5对多态性较强、扩增带型稳定的引物,对江西产区9个不同粉葛栽培种进行遗传多样性分析,共得到16个多态信息位点,平均每个SSR引物多态信息含量(PIC)为0. 600 7;对9个粉葛栽培种进行亲缘关系聚类,结果显示9个粉葛栽培种可归为6个种质。该研究利用5对核心引物组合,为江西粉葛种质建立了DNA身份证,为粉葛优良种质筛选和中药材道地性研究提供参考信息。
The type and frequency of simple sequence repeats( SSRs) in the genomes was investigated using the DNA sequence data of Pueraria lobata and P. thomsonii. Based on these SSRs,20 pairs of SSR primers were designed and 5 high polymorphism primer pairs were selected to analyze genetic diversity of 9 cultivars of P. thomsonii in Jiangxi province. The results showed that the 5 pairs of primers could generate 16 polymorphic alleles bands. The average polymorphism information content( PIC) of each SSR primer pair was 0. 600 7.According to the genetic similarity coefficients,the 9 cultivars of P. thomsonii can be classified into 6 germplasms. This study established DNA identity cards with 5 pairs of SSR primers for different germplasm resources of P. thomsonii in Jiangxi province,which provided reference information for the selection of fine germplasms of P. thomsonii and the theoretical basis for the study of Dao-di herbs.

关键词(KeyWords): 野葛;甘葛藤;基因组SSR;DNA身份证
Pueraria lobata;Pueraria thomsonii;genomic SSR;DNA identity card

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基金项目(Foundation): 国家重点研发计划项目(2017YFC170395-01);; 中央级公益性科研院所基本科研业务费专项资金项目(ZZ10-008);; 中央本级重大支减项目(2060302)

作者(Author): 周荣荣;周骏辉;南铁贵;蒋超;段海燕;赵宇平;黄璐琦;袁媛;
ZHOU Rong-rong;ZHOU Jun-hui;NAN Tie-gui;JIANG Chao;DUAN Hai-yan;ZHAO Yu-ping;HUANG Lu-qi;YUAN Yuan;School of Pharmacy,Nanjing University of Chinese Medicine;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences;

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