万东;祝慧凤;罗勇;谢鹏;徐晓玉;
WAN Dong1,ZHU Hui-feng2,LUO Yong1,XIE Peng1,XU Xiao-yu2(1.Department of Neurology,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;2.Pharmacy College of Chongqing Medical University,Chongqing 400016,China)

• 1:重庆医科大学附属第一医院神经内科
• 2:重庆医科大学药学院
• 3:重庆医科大学附属第一医院神经内科
• 4:重庆医科大学附属第一医院神经内科

摘要(Abstract)：

目的:观察梓醇对原代培养的新生SD大鼠皮质神经元生存活性及轴突生长的影响。方法:新生24 h内SD大鼠大脑皮质神经元原代培养6 d后,分为空白组、终浓度分别为0.25,0.5,1.0,2.5,5.0 mg.mL-1梓醇干预组和终浓度为1.0 mg.mL-1胞磷胆碱阳性药物对照组。药物干预48 h后,观察细胞生长情况,用NF-200免疫组化染色鉴定神经元,MTT法检测神经元生存活性,测微尺测量神经元轴突长度。结果:梓醇终浓度在1⁵ mg.mL-1时,可明显促进神经元轴突生长,2.5 mg.mL-1时作用最强;梓醇各浓度组神经元生存活性与空白组和胞磷胆碱组差异无显著性。结论:梓醇能明显促进皮质神经元轴突生长,但对其生存活性无显著影响。
Objective:To explore the effects of different concentration of catalpol on the cell survival and axonal growth of cortical neurons cultured in vitro from 24 h newly born rat.Method:Primary cultured cortical neurons from 24 h newly born rat were dissociated and cultured.The different concentration of catalpol and 1 mg·mL-1 citicoline were added to the culture plates for 48 h,and the final of catalpol concentration were 0.25,0.5,1,2.5,5 mg·mL-1,respectively.The cortical neuron was identified by NF-200 antigen and its survival activity detected by MTT assay.The axonal growth of cultured cortical neuron were observed by inverted microscopy with micrometer.Result:Immunocytochemistry demonstrated more than 95% of the primary cultured cortical neurons were positive for NF-200 antigen,which indicated the cultured cells were neurons.Neurons survived growing on the concentration of 0.25,0.5,1,2.5,5 mg·mL-1.Compared with blank and 1 mg·mL-1 citicoline group,neurons survival rates were not statistical significant difference.However,it demonstrated that catalpol significantly promoted axonal growth from 1-5 mg·mL-1(P<0.05).Interestedly,compared with the dose of 2.5 mg·mL-1,axonal growth was shorter at the dose of 5 mg·mL-1,and 2.5 mg·mL-1 catalpol showed the strongest promotion effect.Conclusion:The catalpol can enhance cortical neuron axonal growth,but not promote cortical neuron survival.

关键词(KeyWords)： 梓醇;皮质神经元;细胞培养;轴突长度
catalpol;cortical neurons;primary culture;axon length

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30070915);; 重庆市科委应用基础研究项目(渝科发计字[2002]18-99)

作者(Authors): 万东;祝慧凤;罗勇;谢鹏;徐晓玉;
WAN Dong1,ZHU Hui-feng2,LUO Yong1,XIE Peng1,XU Xiao-yu2(1.Department of Neurology,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;2.Pharmacy College of Chongqing Medical University,Chongqing 400016,China)

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