中国中药杂志

2016, v.41(11) 2087-2092

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采收及加工方法对金边蚂蟥抗凝血酶活性影响的研究
Effects of harvest and different processing methods on anti-thrombin activity of Poecilobdella manillensis

勾玲;程搏幸;郭巧生;史红专;刘飞;卢昱希;王嘉;闫士猛;吴曼郡;戴道新;
GOU Ling;CHENG Bo-xing;GUO Qiao-sheng;SHI Hong-zhuan;LIU Fei;LU Yu-xi;WANG Jia;YAN Shi-meng;WU Man-jun;DAI Dao-xin;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;

摘要(Abstract):

研究了采收及不同加工方法对金边蚂蟥抗凝血酶活性的影响规律。分别设置了6种加工方法,4个部位和5个体重等级,采用凝血酶直接滴定法分别对各处理样品进行抗凝血酶活性测定。结果表明,在加工方法中,金边蚂蟥鲜品经冷冻干燥处理后其抗凝血酶活性最高,达1 303.56 U·g~(-1),而90℃烘干样品最低,为15.44 U·g~(-1);在不同部位中,金边蚂蟥头部的抗凝血酶活性最高,为226.42 U·g~(-1),尾部仅为102.12 U·g~(-1);在不同体重级别中,金边蚂蟥体重≤10 g时,抗凝血酶活性最高,为328.86 U·g~(-1),体重≥40 g时,抗凝血酶活性最低,为87.71 U·g~(-1)。综上所述,采收及不同加工方法对金边蚂蟥抗凝血酶活性均有显著性影响。在金边蚂蟥加工中应该选取体质量在20~30 g的金边蚂蟥,优先选用超低温冷冻干燥方法进行干燥,其次选用晒干方法进行干燥。
The effects of harvest and different processing methods on the anti-thrombin activity of Poecilobdella manillensis were respectively studied. The indicators included processing methods( vacuum freeze drying,fresh homogenate,drying under sunlight,freezing,scalding,baking under different temperatures),different parts( entire body,cephalon,pygidium,exudate) and body weights( ≤10,10-20,20-30,30-40,≥40 g). The anti-thrombin activities of P. manillensis with different processing methods were evaluated by direct anti-thrombin titration. The results indicated that the processing methods significantly affected the anti-thrombin activities of P. manillensis. Among the 11 groups,the anti-thrombin activity of P. manillensis processed with vacuum freeze drying( 1 303. 56 U·g~(-1)) was significantly highest than the other groups( P < 0. 05),and that processed with baking under 90 ℃( 15. 44 U·g~(-1)) was the lowest. The anti-thrombin activity of the cephalon of P. manillensis( 226. 42 U·g~(-1)) was the highest,and that of the pygidium( 102. 12 U·g~(-1)) was lowest; the anti-thrombin activities for different body weights were significantly different( P < 0. 05); and among the five groups,the body weight of ≤10 g( 328. 86 U · g~(-1)) was the highest( P < 0. 05),and the body weight of ≥40 g( 87. 71 U·g~(-1)) was the lowest. In conclusion,harvest and different processing methods had a significant impact on the anti-thrombin activities of P. manillensis. In the study,for the optimal processing method for P. manillensis,the body weight between 20-30 g is recommended,and the vacuum freeze drying is preferred,which is followed by the drying under sunlight.

关键词(KeyWords): 金边蚂蟥;采收;加工方法;抗凝血酶活性
Poecilobdella manillensis;harvest;different processing methods;anti-thrombin activity

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81473306,81503186);; 中国博士后科学基金面上项目(2014M551614);; 江苏省博士后科研项目(1302032C)

作者(Author): 勾玲;程搏幸;郭巧生;史红专;刘飞;卢昱希;王嘉;闫士猛;吴曼郡;戴道新;
GOU Ling;CHENG Bo-xing;GUO Qiao-sheng;SHI Hong-zhuan;LIU Fei;LU Yu-xi;WANG Jia;YAN Shi-meng;WU Man-jun;DAI Dao-xin;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;

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