中国中药杂志

2002, (05)

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虎杖商品药材中白藜芦醇苷的含量测定
Determination of Piceid in Commercial Rhizoma Polygoni Cuspidati by HPLC

王磊,黄澜,张勉,王峥涛
WANG Lei, HUANG Lan, ZHANG Mian, WANG Zheng tao (Department of Pharmacognosy, China Pharmaceutical University,Nanjing, 210038, Jiangsu, China)

摘要(Abstract):

目的 :改善虎杖药材中白藜芦醇苷含量测定的方法 ,并对不同来源商品药材进行测定。方法 :高效液相色谱法 ,C18柱为固定相 ,乙腈 水 (2 0∶80 )作为流动相 ,检测波长为 30 3nm ,流速 1mL·min-1。结果 :白藜芦醇苷在 0 .0 0 6 6~ 0 .0 792 μg·μL-1浓度范围内呈现良好的线性关系 ,相关系数r =0 .9999(n =5 ) ;平均回收率为98.5 % ,RSD为 2 .5 % (n =9)。结论 :该方法简单、准确 ,重现性好。不同来源商品药材测定结果表明 ,白藜芦醇苷含量最高达到 3.6 3% ,最低为 1.2 0 % ,平均含量为 2 .2 3% ,差异并不十分悬殊。该方法可作为虎杖质量控制的方法之一
Objective:To improve a RP HPLC method of determining piceid in commercial Rhizoma Polygoni Cuspidati. Method:HPLC method was used, with C 18 column (250 mm×4.6 mm ), a mixture of CH 3CN H 2O (20∶80) as the mobile phase,and detecting wavelength 303 nm. Result: The linear range was 0.0066~0.0792 μg·μL -1 , r =0.9999 ( n =5). The average recovery rate was 98.5% and RSD was 2.5% ( n =9). Conclusion: The method is simple, accurate and reproducible. The contents of piceid in commercial Rhizoma Polygoni Cuspidati from different areas are variable and the average content is 2.23%.

关键词(KeyWords): HPLC;虎杖;白藜芦醇苷;含量测定
HPLC; commercial Rhizoma Polygoni Cuspidati; piceid; determination

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基金项目(Foundation): 国家重点科技项目 (攻关 )计划 (99 92 9 0 1 3 1)

作者(Author): 王磊,黄澜,张勉,王峥涛
WANG Lei, HUANG Lan, ZHANG Mian, WANG Zheng tao (Department of Pharmacognosy, China Pharmaceutical University,Nanjing, 210038, Jiangsu, China)

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