中国中药杂志

2015, v.40(10) 1994-1998

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地锦草改善2型糖尿病模型KK-Ay小鼠胰岛素抵抗的机制研究
Study of effect of Humifuse Euphorbia Herb on alleviating insulin resistance in type 2 diabetic model KK-Ay mice

王琳琳;富宏;黎巍威;宋芳娇;宋宜祥;于倩;刘庚信;王学美;
WANG Lin-lin;FU Hong;LI Wei-wei;SONG Fang-jiao;SONG Yi-xiang;YU Qian;LIU Geng-xin;WANG Xue-mei;Research Studio of Integrated Traditional Chinese and Western Medicine,Peking University First Hospital;

摘要(Abstract):

研究地锦草对2型糖尿病胰岛素抵抗的作用。将高脂饮食的KK-Ay小鼠,分为4个组,二甲双胍组:灌胃盐酸二甲双胍(250 mg·kg-1);模型组:灌胃等量蒸馏水;地锦草低剂量组:灌胃地锦草1 g·kg-1;地锦草高剂量组:灌胃地锦草2 g·kg-1。另外,以普通饮食的C57BL/6J小鼠作为正常对照组:灌胃等量蒸馏水。5组均连续灌胃8周。实验前后,分别对实验小鼠的体重、空腹血糖以及胰岛素耐量进行检测;胰腺组织切片HE染色,观察胰岛的病理变化;采用ELISA方法检测血清胰岛素,TNF-α,IL-6,脂联素(ADPN)及瘦素(LEP)的水平。结果显示地锦草可降低KK-Ay小鼠的体重、空腹血糖,缓解高胰岛素血症,降低注射胰岛素后的血糖-时间曲线下面积,提高30 min血糖下降率,改善胰岛素抵抗状况;各治疗组胰腺病理形态明显改善;地锦草可降低KK-Ay小鼠血清TNF-α,IL-6及LEP水平,升高血清ADPN水平。该研究证明地锦草可改善KK-Ay小鼠胰岛素抵抗,其机制可能与影响炎症因子及脂肪因子水平有关。
To explore the effect of Humifuse Euphorbia Herb( HEH) on alleviating insulin resistance in type 2 diabetic KK-Ay mice. Totally 40 KK-Ay mice fed with high-fat diet were divided into four groups: the metformin group,the model group,the HEH low-dose group and the HEH high-dose group,and orally administrated with metformin hydrochloride( 250 mg·kg-1),distilled water,humifuse euphorbia herb 1 g·kg-1and 2 g·kg-1. Besides,C57 BL /6J mice with ordinary feed were taken as the normal control group and orally administrated with equal distilled water. The oral administration for the five groups lasted for eight weeks. Before and after the experiment,weight,fasting glucose and insulin tolerance were determined. The morphological changes in pancreas were observed through hematoxylin-eosin( HE) staining on pancreatic tissue sections. The serum insulin,TNF-α,IL-6,adiponectin( ADPN)and leptin( LEP) were detected by ELISA. The results showed that HEH could reduce weight and fasting glucose in KK-Ay mice,alleviate hyperinsulinemia,reduce blood glucose-time AUC,increase 30-min blood glucose decline rate,relieve insulin resistance,significantly ameliorate the pathomorphological changes in pancreas in each group,decrease serum TNF-α,IL-6 and leptin levels in KKAy mice and rise serum ADPN level. This study proved that humifuse euphorbia herb can ameliorate the insulin resistance in KK-Ay mice,and its mechanism may be related to the effect on inflammatory factors and adipocytokines.

关键词(KeyWords): 地锦草;2型糖尿病;胰岛素抵抗;炎症因子;脂肪因子
Humifuse Euphorbia Herb;type 2 diabetes;insulin resistance;inflammatory factor;adipocytokine

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划(973)项目(2009CB523003);; 北京科技计划项目(D131100004713004)

作者(Authors): 王琳琳;富宏;黎巍威;宋芳娇;宋宜祥;于倩;刘庚信;王学美;
WANG Lin-lin;FU Hong;LI Wei-wei;SONG Fang-jiao;SONG Yi-xiang;YU Qian;LIU Geng-xin;WANG Xue-mei;Research Studio of Integrated Traditional Chinese and Western Medicine,Peking University First Hospital;

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