中国中药杂志

2014, v.39(14) 2756-2761

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丁桂儿脐贴辅助治疗对泄泻小儿肠道菌群的影响及其作用特征
Effect of adjuvant therapy with Dingguier umbilical paste on intestinal flora of diarrhea infants and its action characteristics

孙海峰;高彦云;安翠红;李玉兰;段丽君;禹玉洪;任武贤;
SUN Hai-feng;GAO Yan-yun;AN Cui-hong;LI Yu-lan;DUAN Li-jun;YU Yu-hong;REN Wu-xian;College of Chemistry & Chemical Engineering,Shanxi University;Shaanxi Provincial Center for Disease Control and Prevention;Taiyuan Research Institute of Traditional Chinese Medicine;Yabao Pharmaceutical Co.,Ltd;

摘要(Abstract):

贴敷治疗是传统中医药治疗小儿泄泻较为独特的一种治疗方法,在临床实践中应用广泛。目前已有众多研究者从中医机制和临床实践中阐释了该治疗途径的合理性,但其作用机制如何,目前并不明确。该研究即在中医临床医师参与下,以不同泄泻证患儿粪便样本为研究材料,在对证口服中药的基础上,辅以丁桂儿脐贴敷脐治疗,采用自动核糖体内转录间隔序列分析法(automated ribosomal intergenic-spacer analysis,ARISA),研究丁桂儿脐贴辅助治疗对泄泻患儿肠道菌群的影响。结果表明敷脐辅助治疗显著影响着肠道菌群的迁移行为,具体表现在与正常对照间菌群相似性差异程度减少和核心OTUs(operational taxonomic units)及与正常对照共享OTUs数量的增加。此外,丁桂儿脐贴辅助治疗还具有作用持续时间长、缓慢增加菌群OTUs数量等特点。提示丁桂儿脐贴具有促进失衡肠道微生态系统恢复的作用,肠道菌群可能是贴敷治疗小儿泄泻的主要靶点之一,而单纯对证口服中药的主要作用靶点可能不完全是肠道菌群。
The applicator therapy is a unique method to treat infant diarrhea in traditional Chinese medicines and widely applied in clinical practice. Currently,many researchers have proved the rationality of the therapy based on the traditional Chinese medicine mechanism and on the data from clinical practice,but its action mechanism is uncertain at present. In this study,with the assistance of pediatric practitioners,the automated ribosomal intergenic-spacer analysis( ARISA) was adopted to study the effect of the adjuvant therapy with Dingguier umbilical paste on intestinal flora of diarrhea infants,in which Dingguier umbilical paste served as the adjuvant therapy in oral traditional Chinese medicines and fecal samples of infants with different diarrhea symptoms were collected and used as the study materials. The results showed that the adjuvant therapy had a significant effect on the shift of intestinal flora,which was associated with the decrease in the similarity difference to the normal control group and the increase in the number of operational taxonomic units( OTUs) shared with the normal control group. Additionally,adjuvant therapy with Dingguier umbilical paste also showed long action duration and increased OTUs number. These results indicated that Dingguier umbilical paste has the effect in restoring the micro-ecosystem of unbalanced intestinal bacteria. Intestinal flora may be one of major targets for the applicator therapy for the infant diarrhea,but not for the single oral traditional Chinese medicine for infant diarrhea.

关键词(KeyWords): 丁桂儿脐贴;贴敷治疗;小儿泄泻;肠道菌群;作用机制
Dingguier umbilical paste;applicator therapy;infant diarrhea;intestinal flora;action mechanism

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2011ZX09201-201);; 山西省基础研究计划项目(2011011035-5)

作者(Author): 孙海峰;高彦云;安翠红;李玉兰;段丽君;禹玉洪;任武贤;
SUN Hai-feng;GAO Yan-yun;AN Cui-hong;LI Yu-lan;DUAN Li-jun;YU Yu-hong;REN Wu-xian;College of Chemistry & Chemical Engineering,Shanxi University;Shaanxi Provincial Center for Disease Control and Prevention;Taiyuan Research Institute of Traditional Chinese Medicine;Yabao Pharmaceutical Co.,Ltd;

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