中国中药杂志

2010, v.35(22) 3021-3023

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

RP-HPLC法测定八角莲属植物中槲皮素及山柰酚的含量
Determination of quercetin and kaempferol in Dysosma plants by RP-HPLC

罗君;张丽艳;万明香;何顺志;杨玉琴;
LUO Jun1,Zhang Liyan2,WAN Mingxiang1,HE Shunzhi2,YANG Yuqin2(1.First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guiyang 550001,China;2.Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China)

摘要(Abstract):

目的:对不同种、不同药用部位、不同产地的八角莲属植物中槲皮素、山柰酚含量进行对比研究,为合理开发利用八角莲属药材提供理论依据。方法:采用RP-HPLC法;Diamonsil C18色谱柱(4.6 mm×150 mm,5μm);流动相甲醇-0.1%磷酸溶液(60∶40);流速1.0 mL.min-1;检测波长360 nm;柱温25℃。结果:槲皮素及山柰酚的线性范围分别为0.22~1.1,0.42~2.1μg,平均回收率分别为97.1%(RSD 1.4%)及99.6%(RSD 2.4%)。结论:不同种、不同产地的八角莲属植物中黄酮类成分有显著差异。
Objective: To determine quercetin and kaempferol in the plant of genus Dysosma that come from different species,different plant parts or different growing areas,which provide the basis of rational utilization of Dysosma plants.Method: The analysis was performed on a Diamonsil C18 column(4.6 mm×150 mm,5 μm) eluted with the mobile phase of methanol-water containing 0.1% phosphoric acid(60∶40).The flow rate was 1 mL·min-1,the detection wavelength was 360 nm;and the column temperature was set at 25 ℃.Result: The linear ranges of quercetin and kaempferol are 0.22-1.1 μg and 0.42-2.1 μg.The average recoveries of quercetin and kaempferol are 97.1%(RSD 1.4%) and 99.6%(RSD 2.4%);respectively.Conclusion: The contents of flavones in different species of Dysosma are significantly different.

关键词(KeyWords): RP-HPLC;八角莲属植物;槲皮素;山柰酚
RP-HPLC;Dysosma plants;quercetin;kaempferol

Abstract:

Keywords:

基金项目(Foundation): 国家中医药管理局科技项目(04-05ZP59)

作者(Author): 罗君;张丽艳;万明香;何顺志;杨玉琴;
LUO Jun1,Zhang Liyan2,WAN Mingxiang1,HE Shunzhi2,YANG Yuqin2(1.First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guiyang 550001,China;2.Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享