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丹参毛状根化学成分稳定性研究
Chemical stability of Salvia miltirrhiza hairy root

吕冬梅;袁媛;张东;黄璐琦;梁日欣;邱德有;
LV Dong-mei1,YUAN Yuan1,ZHANG Dong,HUANG Lu-qi1,LIANG Ri-xin1,QIU De-you2 (1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Institute of Forestry,Chinese Academy of forestry,Beijing 100091,China)

摘要(Abstract):

目的:研究丹参毛状根的化学物质稳定性,为进一步探索丹参毛状根作为原料药的可行性提供研究基础。方法:PCR扩增10批丹参毛状根的rolA基因,HPLC指纹图谱分析10批丹参毛状根的化学成分稳定性。结果:PCR结果表明,外源基因rolA在10批样品的基因组DNA中均可以检测到;通过对丹参毛状根的指纹图谱研究,10批毛状根的相似度均在0.970以上。结论:不同批次丹参毛状根样品间化学成分的差异不显著。
Objective: To study on the chemical stability of Salvia miltirrhiza hairy root.Method: The rolA gene was detected by PCR in DNA and the chemical contituent variances were detected by HPLC.Result: The rolA gene was found in all the 10 batches of the culfured hairy root.The similarities of the chromatographic fingerprints of the 10 batches are higher than 0.95.Conclusion: There are no significant differences of the chemical constituents in 10 hairy root samples.

关键词(KeyWords): 丹参毛状根;PCR;化学稳定性;HPLC
Salvia miltirrhiza hairy root;chemical stability;HPLC

Abstract:

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基金项目(Foundation): 国家重点基础研究发展计划(2006CB504700);; 国家高新技术研究发展计划(2007AA02Z104)

作者(Author): 吕冬梅;袁媛;张东;黄璐琦;梁日欣;邱德有;
LV Dong-mei1,YUAN Yuan1,ZHANG Dong,HUANG Lu-qi1,LIANG Ri-xin1,QIU De-you2 (1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Institute of Forestry,Chinese Academy of forestry,Beijing 100091,China)

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