中国中药杂志

2014, v.39(20) 3950-3954

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不同炮制方法对加拿大产西洋参中10种人参皂苷的影响
Influence of different processed methods on 10 kinds of ginsensides in Panacis Quinquefolii Radix

黄艳菲;孙美;许云章;王静霞;赵小燕;陈科力;黄必胜;刘圆;
HUANG Yan-fei;SUN Mei;XU Yun-zhang;WANG Jing-xia;ZHAO Xiao-yan;CHEN Ke-li;HUANG Bi-sheng;LIU Yuan;Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription,Hubei University of Traditional Chinese Medicine;Ethnic Medicine Institute of Southwest University for Nationalities;

摘要(Abstract):

探讨不同炮制方法对加拿大产西洋参中10种人参皂苷的影响。采用课题组前期已建立的测定西洋参人参皂苷的HPLC-PDA-ESI-MS方法,测定自制西洋白参(新鲜全参切片+-80℃冷冻干燥)、自制西洋红参(新鲜全参+隔水蒸煮+切片+-80℃冷冻干燥)和购于加拿大西安大略省大山行农场的商品西洋参(新鲜全参+电热鼓风烘干)中10种人参皂苷在炮制前后的变化情况。人参总皂苷含量为:商品西洋参>自制西洋白参>自制西洋红参;与自制西洋白参相比,自制西洋红参人参皂苷Re,Rc,Rb2,Rb3的含量降低,人参皂苷Rg1,Rb1的含量升高;人参皂苷Rg2,Rg3在六年生自制西洋白参、商品西洋参中采用PDA检测器均未检测到,采用ESI-MS能检测到较低响应值的Rg2,Rg3,但是在六年生自制西洋红参中其质量分数分别达到了0.027%,0.040 1%;通过二级质谱分析发现新鲜加拿大西洋参炮制成西洋红参后,人参皂苷RA0的量增加;人参皂苷Rf在所有西洋参样品中均未检测到。炮制后加拿大产西洋红参中人参皂苷的含量发生了较大变化,部分原本含量很低的人参皂苷含量增加,部分人参皂苷含量降低;与文献报道一致,西洋参样品中不含人参皂苷Rf。
The influence on 10 kinds of ginsensides of different processed methods of Panacis Quinquefolii Radix was discussed. White Panacis Quinquefolii Radix( sliced and dried at- 80 ℃),red Panacis Quinquefolii Radix( steamed,sliced and dried at- 80 ℃) and commercial Radix Panacis Quinquefolii( dried by electric blast air) processed by different methods. HPLC-PDA-ESIMS method was established before by our team. Ten kinds of ginsenosides of them were determined. The content of total ginsenosides were as follow: commercial Panacis Quinquefolii Radix > white Panacis Quinquefolii Radix > red Panacis Quinquefolii Radix. Compared with white Panacis Quinquefolii Radix,the content of Re,Rc,Rb3 and Rb2of Red Radix Panacis Quinquefolii decreased but increased that of Rg1,Rb1. Both Rg2 and Rg3were not found in white Panacis Quinquefolii Radix and commercial Panacis Quinquefolii Radix by PDA detector,and low response in ESI-MS,while red Panacis Quinquefolii Radix was to the high content that of 0. 027% and0. 040 1%. The constituent of RA0 of red Panacis Quinquefolii Radix was higher than the other two. After Panacis Quinquefolii Radix processed,the kind and content of ginsensides were significantly changed. The constituent of some kinds of ginsensides was increased and some decreased. Rf was not found in all Panacis Quinquefolii Radix samples which were consistent with the former documents.

关键词(KeyWords): 加拿大;西洋参;炮制,HPLC-PDA-ESI-MS;人参皂苷
Canada;Panacis Quinquefolii Radix;processed;HPLC-PDA-ESI-MS;ginsensides

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81173653);; 国家科技支撑计划项目(2012BAI27B07);; 西南民族大学“两翼”建设研究项目(13NLY01)

作者(Author): 黄艳菲;孙美;许云章;王静霞;赵小燕;陈科力;黄必胜;刘圆;
HUANG Yan-fei;SUN Mei;XU Yun-zhang;WANG Jing-xia;ZHAO Xiao-yan;CHEN Ke-li;HUANG Bi-sheng;LIU Yuan;Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription,Hubei University of Traditional Chinese Medicine;Ethnic Medicine Institute of Southwest University for Nationalities;

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