中国中药杂志

2003, (10)

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醋制延胡索微粉提取条件的研究
Study on Extraction Conditions for Minute Powder of Processed Rhizome of Corydalis with Vinegar

张永欣,张颖,张启伟
ZHANG Yong-xin, ZHANG Ying, ZHANG Qi-wei (Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China)

摘要(Abstract):

目的 :对影响醋制延胡索微粉提取的诸因素进行考察 ,为醋制延胡索微粉的应用奠定基础。方法 :采用单因素试验方法 ,以活性成分延胡索乙素为指标 ,高效液相色谱法测定其含量。DiamonsilC18色谱柱 ;流动相乙腈 三乙胺磷酸盐缓冲溶液 (pH 7.0 ) (5 0∶5 0 ) :检测波长 2 80nm。 结果 :实验得出醋制延胡索微粉提取条件为 :温度为 6 0℃ ,提取时间 30min ,加水量 2 0倍。结论 :微粉的提取效率比饮片煎煮的要高约 10 %。但微粉提取用水量少 ,提取时间短 ,温度低。在工业化生产中可以降低能耗 ,提高经济效益。
Objeetive: To examine the factors in extracting the minute powder of processed rhizome of Corydalis with vinegar for its application. Method: The one-way variation analysis was used for experimental design. Tetrahydropalmatine was determined with HPLC method. The samples were separated on a Diamonsil C 18 column with mobile phase of acetonitrile-TEA phosphate buffer (pH 7.0) (50∶50) and detection wavelength was 280mm. Result: The extraction conditions were established as follows: extraction temperature is 60 ℃, water-added amount is 20 times the weight of the powder, and extraction time is 30 minutes. Conclusion: The extraction rate of the trahydropalmatine in the minute power is about 10% higher than that of the processed rhizome with vinegar. But less water amount, less time, and lower temperature were needed for extraction. The application of minute powder can reduce energy consumption and raise economic benefit in industrial production.

关键词(KeyWords): 醋制延胡索;微粉;提取条件;延胡索乙素;高效液相色谱法
processed rhizome of corydalis with vinegar; minute powder; tetrahydropalmatine; HPLC

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作者(Author): 张永欣,张颖,张启伟
ZHANG Yong-xin, ZHANG Ying, ZHANG Qi-wei (Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China)

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