王晓明;王跃飞;潘桂湘;杨静;
WANG Xiao-ming WANG Yue-fei PAN Gui-xiang YANG Jing (The Traditional Chinese Medicine Research Centre of Tianjin University of Traditional Chinese Medicine,Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae,Ministry of Education,Tianjin 300193,China)

• 1:天津中医药大学中医药研究院省部共建教育部方剂学重点实验室

摘要(Abstract)：

目的:建立同时测定灯盏细辛注射液中绿原酸、咖啡酸、1,5-二-O-咖啡酰奎宁酸、野黄芩苷的含量测定方法。方法:采用高效液相色谱法,Agilent Zorbax SB-C18(4.6 mm×150 mm,5μm)色谱柱;流动相A-0.1%甲酸水溶液,B-乙腈,梯度洗脱;流速1.0 mL·min^(-1),柱温35℃,进样量20μL。结果:绿原酸、咖啡酸、1,5-二-O-咖啡酰奎宁酸、野黄芩苷分别在0.025～0.800(r=0.999 0),0.027～0.850(r=0.999 9),0.062～1.978(r=0.999 7),0.118～3.770μg(r=0.999 9)成良好的线性关系,加样回收率分别为97.19%(RSD 1.16%);100.45%(RSD 1.16%);97.32%(RSD 1.43%);103.81%(RSD 0.70%)。结论:本法简便、快捷,结果准确、重复性好,可用于灯盏细辛注射液中4种有效成分的含量测定。
Objective:To establish a method for determination of chlorogenic acid,caffeic acid,caffeoylquinic acid and Scute- llarin in Dengzhan Xixin injection.Mothod:The HPLC method was carried out on Agilent Zorbax SB-C18 column(150 mm×4.6 mm,5μm)evaluated with acetonitrile-0.1% formic acid as mobile phase,gradient elution;the flow rate was 1.0 mL·min(-1),柱温35℃,进样量20μL。结果:绿原酸、咖啡酸、1,5-二-O-咖啡酰奎宁酸、野黄芩苷分别在0.025～0.800(r=0.999 0),0.027～0.850(r=0.999 9),0.062～1.978(r=0.999 7),0.118～3.770μg(r=0.999 9)成良好的线性关系,加样回收率分别为97.19%(RSD 1.16%);100.45%(RSD 1.16%);97.32%(RSD 1.43%);103.81%(RSD 0.70%)。结论:本法简便、快捷,结果准确、重复性好,可用于灯盏细辛注射液中4种有效成分的含量测定。
Objective:To establish a method for determination of chlorogenic acid,caffeic acid,caffeoylquinic acid and Scute- llarin in Dengzhan Xixin injection.Mothod:The HPLC method was carried out on Agilent Zorbax SB-C18 column(150 mm×4.6 mm,5μm)evaluated with acetonitrile-0.1% formic acid as mobile phase,gradient elution;the flow rate was 1.0 mL·min^(-1);the temperatue of column was at 35℃;the detection wavelength was at 335 nm for UV detection.Result:The calibration curves were lin- ear in the range of 0.025 to 0.800μg(r=0.999 0)for chlorogenic acid,0.027 to 0.850μg(r=0.999 9)for caffeic acid,0.062 to 1.978μg(r=0.999 7)for caffeoylquinic acid and 0.118 to 3.770μg(r=0.999 9 )for scutellarin,respectilely.The average recov- eries were 97.19% with RSD 1.16%;100.45% with RSD 1.16% ;97.32% with RSD 1.43% and 103.81% with RSD 0.70%,re- spectively.Conclusion:The assay demonstrated that the method was simple,it had adequate accuracy and selectivity to quantify the four active components in Dengzhan Xixin injection.

关键词(KeyWords)： 灯盏细辛注射液;绿原酸;咖啡酸;1，5-二-O-咖啡酰奎宁酸;野黄芩苷
Dengzhan Xixin injection;chlorogenic acid;caffeic acid;caffeoylquinic acid;scutellarin

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展规划项目(2005CB523404)

作者(Authors): 王晓明;王跃飞;潘桂湘;杨静;
WANG Xiao-ming WANG Yue-fei PAN Gui-xiang YANG Jing (The Traditional Chinese Medicine Research Centre of Tianjin University of Traditional Chinese Medicine,Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae,Ministry of Education,Tianjin 300193,China)

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