中国中药杂志

2015, v.40(19) 3770-3774

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不同蒸制时间对人参活性成分及其药代动力学行为与抗炎作用的影响研究
Active ingredients and its pharmacokinetic behavior and anti-inflammatory effects of ginseng with different steamed times

钱静;康安;狄留庆;狄亚维;李杰;刘廷;
QIAN Jing;KANG An;DI Liu-qing;DI Ya-wei;LI Jie;LIU Ting;College of Pharmacy,Nanjing University of Chinese Medicine;Jiangsu Engineering Research Center for Efficient Delivery System of Traditional Chinese Medicine;Nanjing Engineering and Technology Research Center for Traditional Chinese Medicine Pellets;

摘要(Abstract):

选用优质人参分别高压蒸制0,2,4,8 h后制备人参提取物,并利用HPLC研究人参蒸制前后的化学成分发生的变化;采用UFLC-MS/MS多反应监测(MRM)定量分析,以地高辛为内标物在负离子模式条件下,研究灌服不同蒸制时间的人参提取物的小鼠体内人参皂苷类成分的药代动力学行为差异;以及通过给灌胃人参提取物1周后的小鼠腹腔注射脂多糖(LPS),建立炎症模型,运用酶联免疫法检测炎症模型小鼠血浆中的肿瘤坏死因子α(TNF-α)和白细胞介素-1β(IL-1β)的含量,探讨不同蒸制时间对人参发挥抗炎作用的影响。经测定灌服蒸制8 h的人参提取物可明显降低炎症模型小鼠血浆中的TNF-α和IL-1β含量。研究结果表明人参经蒸制后化学成分发生变化,入血成分相应改变,人参经蒸制8 h后有助于起到抗炎效果,为进一步揭示人参发挥抗炎作用的物质基础和量效关系提供实验依据。
HPLC analysis was performed to study the changes in chemical composition of ginseng extracts prepared from high quality ginseng with 0,2,4,8 h of steamed times. An UFLC-MS / MS multiple-reaction monitoring( MRM) quantitative analysis was made to investigate the pharmacokinetic behavior differences of ginsenosides in mice ig administered of ginseng extracts with different steamed times in the negative ion mode,with Digoxin as the internal standard substance. The mice were injected with LPS to establish inflammation model after ig administration of ginseng for a week and the contents of tumor necrosis factor-α( TNF-α) and interleukin-1β( IL-1β) in mice plasma were detected by ELISA,in order to study on anti-inflammatory effects of ginseng with different steamed times. It was determined that levels of TNF-α and IL-1β were significantly decreased in inflammation model group ig administered of ginseng extracts with 8h of steamed time. The results showed that the chemical components in ginseng changed after steaming and the components into the blood changed,correspondingly. Ginseng with steamed 8 h contributes to anti-inflammatory effects. These results provided an experimental basis for revealing the active substance basis and dose-effect relationship of ginseng on anti-inflammatory effect.

关键词(KeyWords): 人参;蒸制;人参皂苷;TNF-α;IL-1β
ginseng;steaming;ginsenoside;TNF-α;IL-1β

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基金项目(Foundation): 国家自然科学基金项目(81202983);; 江苏高校优势学科建设工程项目(PAPD);; 江苏省“青蓝工程”创新团队支持计划项目(QLP)

作者(Author): 钱静;康安;狄留庆;狄亚维;李杰;刘廷;
QIAN Jing;KANG An;DI Liu-qing;DI Ya-wei;LI Jie;LIU Ting;College of Pharmacy,Nanjing University of Chinese Medicine;Jiangsu Engineering Research Center for Efficient Delivery System of Traditional Chinese Medicine;Nanjing Engineering and Technology Research Center for Traditional Chinese Medicine Pellets;

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