中国中药杂志

2005, (16) 1265-1268

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反相高效液相色谱法测定牡丹皮中2种活性成分
Determination of two main components in bark of Paeonia Suffruticosa by HPLC

田元子,王琰,刘建祯,杨蓓蓓,石建功,王慕邹
TIAN Yuan-zi, WANG Yan, LTU Jian-zhen, YANG Bei-bei, SHI Jian-gong, WANG Mu-zou (Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China)

摘要(Abstract):

目的:建立HPLC测定牡丹皮中2种活性成分丹皮酚和没食子酸的含量。方法:采用AlltimaC18色谱柱(4.6mm×150mm,5μm),以水-四氢呋喃-甲醇-冰醋酸(60:20:20:0.5)为流动相,流速0.8mL·min-1,检测波长274nm。结果:丹皮酚、没食子酸的线性范围分别为0.16-2.58μg(r=0.9999,n=3),0.06-1.0μg(r=0.9999,n=3),平均回收率(n=9)分别为98.2%(RSD2.5%),98.6%(RSD3.0%)。提取方法为甲醇冷浸24h。结论:本方法准确、简捷,可为评价不同产地的牡丹皮质量提供依据。
Objective: To establish a HPLC method for determination two constituents in bark of Paeonia, Suffruticosa. Method: The reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm ×150 mm,5 μm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL·min-1 and UV detection was set at 274 nm. Result: The assay displayed good linearity over the concentration ranges of 0.06-1.0 μg (r=0.999 9, gallic acid) and 0.16-2.58 μg(r =0.9999, paeonol) respectively. The average recoveries (n=9) of gallic acid and paeonol were 98.6% (RSD=3.0%),98.2%(RSD=2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration. Conclusion: The method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.

关键词(KeyWords): 牡丹皮;丹皮酚;没食子酸;高效液相色谱法
HPLC; Cortex Moutan; paeonol; gallic acid

Abstract:

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基金项目(Foundation): 国家科技项目(攻关)计划资助(99-929-01-26)

作者(Author): 田元子,王琰,刘建祯,杨蓓蓓,石建功,王慕邹
TIAN Yuan-zi, WANG Yan, LTU Jian-zhen, YANG Bei-bei, SHI Jian-gong, WANG Mu-zou (Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China)

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