中国中药杂志

2004, (09)

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四物汤对放射线致血虚证小鼠骨髓蛋白质表达的影响
Effects of Siwu Tang on protein expression of bone marrow of blood deficiency mice induced by irradiation

郭平,马增春,李鹰飞,梁乾德,王继峰,王升启
GUO Ping 2, MA Zeng-chun 1, LI Ying-fei 1, LIANG Qian-de 1, W ANG Ji-feng 2, WANG Sheng-qi 1(1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Bei jing 100850, China; 2. Beijing University of Chinese Medicine, Beijing 100029, China) [

摘要(Abstract):

目的 :观察四物汤对血虚证小鼠骨髓蛋白质表达的影响 ,为阐明四物汤补血作用的分子机理提供理论和实验依据。方法 :采用 3.5Gy60Coγ射线全身 1次性照射 ,制备小鼠血虚证模型。利用双向电泳、图像分析、胶内酶切、质谱鉴定等蛋白质组学技术 ,结合生物信息学 ,分离、分析、鉴定蛋白质。结果 :四物汤可以逆转放射线致血虚证小鼠骨髓 10个上调和 5个下调的蛋白质。其中 8个蛋白质可能分别是淋巴细胞特异性蛋白质 1、蛋白酶体2 6SATP酶亚组分 4、造血细胞蛋白质酪氨酸磷酸酶、H ras、3 磷酸甘油醛脱氢酶、生长因子受体结合蛋白 14及Lgals12。结论 :四物汤能调节骨髓蛋白质表达 ,并可能由此促进造血细胞的生长和分化 ,发挥补血作用。
Objective: To observe the effects of Siwu T ang on protein expression of bone marrow of blood deficiency mice and provide th e theoretical and experimental basis for understanding the molecular mechanism o f blood enriching function of Siwu Tang. Method: Blood de ficiency mice were established by using 3.5 Gy 60 Co γ-ray. With proteomic technologies including two-dimensional electrophoresis, image analysi s, in-gel digestion, peptide mass fingerprinting and bioinformatics the protein s of bone marrow of blood deficiency mice were isolated, analyzed, and identifie d. Result: Siwu Tang could reverse 10 up-regulated and 4 down-regulated proteins of blood deficiency mice bone marrow. Seven of the pro teins were likely to be lymphocyte specific protein 1, proteasome 26S ATPase sub u nit 4, hematopoietic cell protein-tyrosine phosphatase, glyceraldehyde-3-phos phate dehydrogenase, growth factor receptor binding protein 14 and lgals12 respe ctively. Conclusion: Siwu Tang can regulate the protein e xpression of bone marrow of blood deficiency mice and thus promote the growth an d differentiation of hematopoietic cells and then exert its effects on blood enr iching function.[

关键词(KeyWords): 四物汤;辐射;血虚证;功能蛋白质组学;骨髓
Siwu Tang; irradiation; blood deficiency; functional proteomics; bone morrow

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目 ( 3 0 2 71617,3 0 0 70 913 ) ;; 军队“十五”重点项目 ( 0 1Z0 19) ;; 北京市“二四八”重大创新工程项目(H0 10 2 10 2 2 0 113 )

作者(Authors): 郭平,马增春,李鹰飞,梁乾德,王继峰,王升启
GUO Ping 2, MA Zeng-chun 1, LI Ying-fei 1, LIANG Qian-de 1, W ANG Ji-feng 2, WANG Sheng-qi 1(1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Bei jing 100850, China; 2. Beijing University of Chinese Medicine, Beijing 100029, China) [

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