中国中药杂志

2015, v.40(13) 2559-2564

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中国川续断属药用种遗传多样性与亲缘关系的SRAP分析
Analysis on genetic diversity and genetic relationship of medicinal species in Dipsacus from China by SRAP

陈大霞;张雪;王钰;李隆云;张泽;
CHEN Da-xia;ZHANG Xue;WANG Yu;LI Long-yun;ZHANG Ze;Chongqing Academy of Chinese Materia Medica;Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica;Chongqing Key Laboratory of Chinese Medicine Resources;Chongqing Sub-center of National Resource,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;College of Life Sciences,Chongqing University;

摘要(Abstract):

采用SRAP分子标记技术分析我国8个川续断属药用种的遗传多样性和亲缘关系,应用POPGENE计算遗传参数,TREECONW软件进行聚类分析。结果表明:26对引物共检测到558条带,其中多态性条带539条;种间的PPB=96.59%,Na=1.965 9,Ne=1.337 5,H=0.214 3,I=0.342 3,表明8个川续断属药用种的遗传多样性较高;种内各遗传参数的平均值为PPB=6.97%,Na=1.069 7,Ne=1.031 1,H=0.018 7,I=0.029 1,表明种内遗传多样性较低;Nei's基因多样性指数计算的遗传分化系数(Gst=0.912 6)说明大部分遗传变异存在于种间;聚类结果与传统经典分类的吻合度极高,且同种的不同植株均聚在一起,证实采用SRAP分子标记进行川续断属药用种的快速鉴别是可靠的,可作为川续断属植物亲缘关系研究的有效方法之一。
The author detected the genetic diversity and genetic relationship within and among eight medicinal species of Dipsacus by the approach of sequence-related amplified polymorphism( SRAP). The associated genetic parameters were calculated by POPGENE1. 31. The Genetic distance was calculated by TREECONW and the systematic diagrams of genetic relationship were clustered by UPGMA. The results showed that,using 26 primers,a total of 558 bands were produced,of which 539 were polymorphic loci. There was a high level of genetic diversity among species( PPB = 96. 59%,Na= 1. 965 9,Ne= 1. 337 5,H = 0. 214 3,I = 0. 342 3). However,genetic diversity was lower within species,the average of genetic parameters was PPB = 6. 97%,Na= 1. 069 7,Ne= 1. 031 1,H =0. 018 7,I = 0. 029 1. The Nei' s genetic differentiation coefficient was 0. 912 6,indicated that most of the genetic variation existed among species. By clustering analysis,different individuals gathered in the same group and the classified result of SRAP marker between traditional modal characters was almost same. The results confirmed that SRAP marker can be used as one of the effective methods to reveal the genetic diversity and relationship among medicinal species of Dipsacus.

关键词(KeyWords): 川续断属;SRAP;药用种;亲缘关系
Dipsacus;SRAP;medicinal species;relationship

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基金项目(Foundation): 国家“十一五”科技支撑计划项目(2006BAI06A11-09);; 国家科技部科技人员服务企业行动项目(2009GJF10045);; 重庆市“科技平台与基地建设”项目(cstc2014ptyjd10001)

作者(Author): 陈大霞;张雪;王钰;李隆云;张泽;
CHEN Da-xia;ZHANG Xue;WANG Yu;LI Long-yun;ZHANG Ze;Chongqing Academy of Chinese Materia Medica;Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica;Chongqing Key Laboratory of Chinese Medicine Resources;Chongqing Sub-center of National Resource,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;College of Life Sciences,Chongqing University;

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