中国中药杂志

2011, v.36(10) 1301-1304

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

大鼠灌胃白花蛇舌草有效部位提取物血浆中代谢产物的液质分析
HPLC-TOF-MS analysis of metabolites of Diffusa effective extracts in rats

晏利芝;王英锋;余启荣;
YAN Lizhi1,WANG Yingfeng2,YU Qirong3(1.Pony Testing Technology Co.,Ltd.,Beijing 100080,China;2.Analysis and Center Capital Normal University,Beijing 100037,China;3.Beijing Supertrack Medical Technology Develop Ltd.,Beijing 100029,China)

摘要(Abstract):

目的:分析大鼠灌胃白花蛇舌草有效部位提取物后血浆中对香豆酸、反式6-O-对香豆酰鸡屎藤苷甲酯及其代谢产物。方法:取健康雄性SD大鼠按0.4 g.kg-1的剂量经口给予白花蛇舌草有效部位提取物,1.0 h后采集眼眶静脉血液制备血浆,采用HPLC-Q-TOF-MS进行分析。结果:在大鼠血浆中只检测到1个代谢产物,并利用对照品鉴定了其结构为对香豆酸。结论:白花蛇舌草有效部位提取物中主要成分反式6-O-对香豆酰鸡屎藤苷甲酯在大鼠体内的代谢产物为对香豆酸,本实验为研究白花蛇舌草化学成分及药效作用提供了理论依据。
Objective: To analyze p-coumaril acid,trans-6-O-p-coumaroyl scandoside methyl ester and its metabolites in rat plasma after intragastric administration of exocarpium Diffusa effective extracts.Method: Rat blood samples were collected 1.0 h after oral administration of 0.4 g·kg-1 exocarpium Diffusa effective extracts,and then were analyzed by using HPLC-Q-TOF-MS.Result: In rat plasma,only one metabolite was detected.The structure was identified by reference substance to be p-coumaril acid.Conclusion: The methyl glucoside metabolite in rat from the main component parts of trans-6-O-p-coumaroyl scandoside methyl ester droppings of vines in Diffusa effective extracts is p-coumaril acid.This experiment provides a theortical basis for studying chemical compositions and pharmacodynamic action of Diffusa.

关键词(KeyWords): 白花蛇舌草有效部位提取物;代谢产物;对香豆酸;HPLC-Q-TOF/MS
Diffusa effective extracts;metabolites;p-coumaril acid;HPLC-Q-TOF/MS

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 晏利芝;王英锋;余启荣;
YAN Lizhi1,WANG Yingfeng2,YU Qirong3(1.Pony Testing Technology Co.,Ltd.,Beijing 100080,China;2.Analysis and Center Capital Normal University,Beijing 100037,China;3.Beijing Supertrack Medical Technology Develop Ltd.,Beijing 100029,China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享