中国中药杂志

2016, v.41(19) 3655-3660

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

淫羊藿苷通过影响MAPK信号通路抑制ox-LDL诱导的血管平滑肌细胞增殖作用研究
Effects of icariin on proliferation of vascular smooth muscle cell induced by ox-LDL via impacting MAPK signaling pathway

胡彦武;李海涛;刘凯;闫梦彤;张洋;任立群;
HU Yan-wu;LI Hai-tao;LIU-Kai;YAN Meng-tong;ZHANG Yang;REN Li-qun;School of Pharmaceutics and Food Science,Tonghua Normal University;Affiliated Hospital of Changchun University of Traditional Chinese Medicine;School of Pharmacy,Jilin University;

摘要(Abstract):

研究淫羊藿苷(ICA)对氧化低密度脂蛋白(ox-LDL)诱导血管平滑肌细胞(VSMC)增殖的抑制作用,以及对增殖细胞核抗原(PCNA)表达和丝裂原活化蛋白激酶(MAPK)信号通路的影响。以ox-LDL(50 mg·L-1)诱导VSMC增殖,采用MTT法检测ICA对ox-LDL诱导VSMC增殖的抑制作用;Real-time PCR和Western blot方法检测ICA对ox-LDL诱导增殖VSMC内细胞周期相关蛋白PCNA基因及蛋白表达的影响;Western blot方法检测ICA对ox-LDL诱导增殖VSMC内p38MAPK,ERK1/2,JNK信号通路相关信号蛋白磷酸化的影响。结果显示,经ox-LDL(50 mg·L-1)刺激后,VSMC增殖活性显著增强,细胞存活率显著提升,S期、G2/M期细胞显著增加,G0/G1期细胞显著减少;同时,PCNA基因及蛋白表达水平显著提升;不同浓度的ICA(40,20,10μmol·L-1)可显著抑制ox-LDL诱导的VSMC增殖,减少S期、G2/M期细胞,增加G0/G1期细胞,降低ox-LDL诱导的PCNA基因及蛋白的表达水平,下调ox-LDL诱导的p-p38和p-ERK1/2蛋白的表达,但不影响p-JNK蛋白的表达。上述结果表明,ICA对ox-LDL诱导的VSMC增殖具有抑制作用,可能通过降低PCNA表达和阻断p38MAPK和ERK1/2信号通路抑制VSMC增殖。
This paper was aimed to study the effects of icariin( ICA) on the proliferation of vascular smooth muscle cell( VSMC)induced by oxidized low density lipoprotein( ox-LDL),and the molecular mechanism of the expression of proliferating cell nuclear antigen( PCNA) and MAPK signaling pathway. In this study,VSMC was induced by ox-LDL( 50 mg·L-1),the effect of ICA on the proliferation of VSMC was detected by MTT assay,Western blot and Real-time PCR. The results showed that after stimulation of ox-LDL,the proliferation activity of VSMC was increased,S phase,G2/M phase cells were increased,G0/G1 phase cells were decreased,PCNA protein expression was enhanced; ICA( 40,20,10 μmol·L-1) could effectively inhibit ox-LDL-induced VSMC proliferation,S phase and G2/M phase cells were decreased,the percentage of cells in G0/G1 phase were increased,PCNA expression was decreased,p38 MAPK and ERK1/2 activation were inhibited. These results indicate that ICA can inhibit the proliferation of VSMC by reducing the expression of PCNA and blocking the p38 MAPK and ERK1/2 signaling pathway.

关键词(KeyWords): 淫羊藿苷;血管平滑肌细胞;增殖细胞核抗原;MAPK信号通路;细胞周期
icariin;vascular smooth muscle cell;proliferating cell nuclear antigen;MAPK signal pathway;cell cycle

Abstract:

Keywords:

基金项目(Foundation): 吉林省自然科学基金项目(20150101221JC);; 吉林省教育厅“十三五”科学技术项目(吉教科合字[2016]第247号);; 通化师范学院应用方向研究项目(2014096)

作者(Authors): 胡彦武;李海涛;刘凯;闫梦彤;张洋;任立群;
HU Yan-wu;LI Hai-tao;LIU-Kai;YAN Meng-tong;ZHANG Yang;REN Li-qun;School of Pharmaceutics and Food Science,Tonghua Normal University;Affiliated Hospital of Changchun University of Traditional Chinese Medicine;School of Pharmacy,Jilin University;

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享