中国中药杂志

2015, v.40(15) 2964-2969

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罂粟属植物核心DNA条形码的筛选
Screening potential DNA barcode regions of genus Papaver

张爽;刘宇婧;吴沿胜;曹颖;袁媛;
ZHANG Shuang;LIU Yu-jing;WU Yan-sheng;CAO Ying;YUAN Yuan;College of Life and Environmental Sciences,Minzu University of China;School of Agricultural Equipment and Engineering,Jiangsu University;State Key Laboratory of Dao-di Herbs,China Academy of Chinese Medical Sciences;

摘要(Abstract):

DNA条形码(DNA barcoding)技术是一种准确、高效且对操作人员要求不高的物种鉴定技术。为了筛选出适合罂粟属的DNA条形码,从GenBank上获得罂粟属植物共69条序列,包括21条ITS序列,10条mat K序列,8条psb A-trnH序列,14条rbcL序列和16条trnL-trnF序列。应用Mega 6.0软件分析了罂粟属的ITS,matK,psbA-trnH,rbcL,trnL-trnF序列的特征,通过计算序列的种内、种间距离,评估序列的Barcoding Gap和构建NJ,UPGMA系统发育树进行分析。分析结果表明:trnL-trn F不仅种内变异和种间变异差别最大,而且有明显的barcoding gap,种内变异和种间变异重合较少,能较好地区分不同种类的罂粟;所构建的NJ和UPGMA系统发育树,也能将全部物种区分开。综合考虑,建议把trnL-trnF作为鉴定罂粟属植物的核心条形码,结合其他片段作为组合条形码。
DNA barcoding is an effective technique in species identification. To determine the candidate sequences which can be used as DNA barcode to identify in Papaver genus,five potential sequences( ITS,mat K,psb A-trn H,rbcL,trnL-trnF) were screened.69 sequences were downloaded from Genbank,including 21 ITS sequences,10 matK sequences,8 psb A-trnH sequences,14 rbcL sequences and 16 trnL-trnF sequences. Mega 6. 0 was used to analysis the comparison of sequences. By the methods of calculating the distances in intraspecific and interspecific divergences,evaluating DNA barcoding gap and constructing NJ and UPMGA phylogenetic trees. The sequence trnL-trnF performed best. In conclusion,trnL-trnF can be considered as a novel DNA barcode in Papaver genus,other four sequences can be as combination barcode for identification.

关键词(KeyWords): DNA barcoding;罂粟属;ITS;matK;psbA-trnH;rbcL;trnL-trnF
DNA barcoding;Papaver;ITS;matK;psbA-trnH;rbcL;trnL-trnF

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基金项目(Foundation): 中医药行业专项(201407003)

作者(Author): 张爽;刘宇婧;吴沿胜;曹颖;袁媛;
ZHANG Shuang;LIU Yu-jing;WU Yan-sheng;CAO Ying;YUAN Yuan;College of Life and Environmental Sciences,Minzu University of China;School of Agricultural Equipment and Engineering,Jiangsu University;State Key Laboratory of Dao-di Herbs,China Academy of Chinese Medical Sciences;

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