扶芳藤药材质量标准研究Research on quality standards of Euonymus fortunei
闫利华;金艳;冯学峰;张启伟;申强;刘晓谦;朱辉;张姗姗;王智民;
YAN Li-hua;JIN Yan;FENG Xue-feng;ZHANG Qi-wei;SHEN Qiang;LIU Xiao-qian;ZHU Hui;ZHANG Shan-shan;WANG Zhi-min;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Changzhi Medical College;
摘要(Abstract):
建立扶芳藤药材质量标准,为控制该药材的质量提供实验依据。采用经验、显微鉴别法对该药材性状、显微特征进行描述;参照2010年版《中国药典》附录相关方法,对该药材水分、总灰分、酸不溶性灰分、醇溶性浸出物进行测定;采用薄层色谱法,分别以卫矛醇和对照药材为对照,对该药材进行定性鉴别;在成分分布和组成分析的基础上,以苷元槲皮素和山柰素为指标,通过系统的方法学考察,建立了药材总黄酮醇苷的含量测定方法。含量测定方法采用C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.1%甲酸溶液(51∶49)为流动相,在366 nm波长下对槲皮素和山柰素进行定量分析,再根据换算系数计算总黄酮醇苷的含量。该药材性状、显微、薄层鉴别方法具有较强的专属性,水分平均为8.76%,总灰分平均为6.48%,酸不溶性灰分平均为0.31%,醇溶性浸出物平均为17.48%,总黄酮醇苷含量平均为0.211%。根据实验结果建立了扶芳藤药材质量标准,所建立的质量标准可用于扶芳藤药材的质量控制。
To establish quality standards of Euonymus fortunei,and supply scientific evidence for the quality control of Euonymus fortunei. Empirical and microscopic identification methods were adopted to observe morphological and histological characters. The contents of water,total ash,acid-insoluble ash and alcohol-soluble extractive were analysed according to the methods of Chinese Pharmacopoeia( 2010). Dulcitol and reference herbs were used to identify materia medica of Euonymus fortunei by TLC method. The total flavonol glycosides contents were analysed by HPLC method,using quercetin and kaempferol as reference substances. Quercetin and kaempferol were separated on a C18column( 4. 6 mm × 250 mm,5 μm) with methanol-0. 1% formic acid( 51∶ 49) as the mobile phase and detected at 366 nm. The flavonoid aglycones content was then multiplied by a conversion coefficient,and the result was the total flavonol glycosides content. The macroscopical identification,microscopic features and TLC methods were proper. The average contents of water,total ash,acid-insoluble ash,alcohol-soluble extractive and total flavonol glycosides were 8. 76%,6. 48%,0. 31%,17. 48% and 0. 211%,respectively. The quality standards established on the basis of the research results were suitable for the quality evaluation of Euonymus fortunei.
关键词(KeyWords):
扶芳藤;质量标准;卫矛醇;槲皮素;山柰素;总黄酮醇苷
Euonymus fortunei;quality standards;dulcitol;quercetin;kaempferol;total flavonol glycosides
基金项目(Foundation): 国家“重大新药创制”科技重大专项(2012ZX09304005002);; 国家药典委员会标准提高暨2015版《中国药典》科研任务
作者(Authors):
闫利华;金艳;冯学峰;张启伟;申强;刘晓谦;朱辉;张姗姗;王智民;
YAN Li-hua;JIN Yan;FENG Xue-feng;ZHANG Qi-wei;SHEN Qiang;LIU Xiao-qian;ZHU Hui;ZHANG Shan-shan;WANG Zhi-min;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Changzhi Medical College;
参考文献(References):
- [1]国家中医药管理局.中华本草.第5册[M].上海:上海科学技术出版社,1995:182.
- [2]广东省食品药品监督管理局.广东省中药材标准.第1册[M].广州:广东科技出版社,2004,107.
- [3]李鹏,李航.扶芳藤中的三萜化合物研究[J].中国药学杂志,2000,35(12):847.
- [4]Ouyang X L,Wei L X,Fang X M,et al.Flavonoid constituents of Euonymus fortunei[J].Chem Nat Compd,2013,49(3):428.
- [5]瞿发林,丁青龙,张汉民.扶芳藤化学成分研究[J].南京军医学院学报,2001,23(4):221.
- [6]Yang Y D,Yang G Z,Liao M C,et al.Three new sesquiterpene pyridine alkaloids from Euonymus fortunei[J].Helv Chim Acta,2011,94(6):1139.
- [7]周小雷,王硕,袁经权,等.瑶药扶芳藤的研究现状[J].现代中药研究与实践,2011,25(3):79.
- [8]广西壮族自治区卫生厅.广西中药材标准.第2册[S].1996:112.
- [9]叶勇,覃洁萍,李芸,等.不同来源扶芳藤药材的HPLC指纹图谱比较[J].广西中医学院学报,2005,8(4):53.
- [10]Ouyang X L,Fang X M,Pan Y M,et al.Development of HPLC fingerprint for the quality control of Euonymus fortuneiand distinguishing it from related species[J].Acta Chromatographica,2012,24(2):301.
- [11]覃洁萍,姚蓉,李芸,等.HPLC-ELSD法测定扶芳藤中卫矛醇的含量[J].广西中医药,2008,31(4):58.
- [12]辛华,李建华,陈勇,等.气相色谱法测定不同产地扶芳藤中卫矛醇含量[J].广西科学院学报,2006,22(S):449.
- [13]郑娟梅,毛晓丽,李自达,等.FTIR定量测定扶芳藤中卫矛醇的方法研究[J].中国实验方剂学杂志,2012,18(6):60.
- [14]Hasler A,Sticher O.Identification and determination of the flavonoids from Ginkgo biloba by high-performance liquid chromatography[J].J Chromatogr,1992,605:41.
- [15]朱辉.扶芳藤的化学成分研究[D].南昌:江西中医学院,2012.
- [16]朱学鑫,蒋福升,丁志山.槲皮苷生物活性的研究进展[J].蛇志,2012,24(1):47.
- [17]杨秀伟,张建业,徐嵬,等.山柰苷的人肠内细菌生物转化研究[J].药学学报,2005,40(8):717.
- 扶芳藤
- 质量标准
- 卫矛醇
- 槲皮素
- 山柰素
- 总黄酮醇苷
Euonymus fortunei - quality standards
- dulcitol
- quercetin
- kaempferol
- total flavonol glycosides
- 闫利华
- 金艳
- 冯学峰
- 张启伟
- 申强
- 刘晓谦
- 朱辉
- 张姗姗
- 王智民
YAN Li-hua- JIN Yan
- FENG Xue-feng
- ZHANG Qi-wei
- SHEN Qiang
- LIU Xiao-qian
- ZHU Hui
- ZHANG Shan-shan
- WANG Zhi-min
- Institute of Chinese Materia Medica
- China Academy of Chinese Medical Sciences
- National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines
- National Resource Center for Chinese Materia Medica
- China Academy of Chinese Medical Sciences
- Changzhi Medical College
- 闫利华
- 金艳
- 冯学峰
- 张启伟
- 申强
- 刘晓谦
- 朱辉
- 张姗姗
- 王智民
YAN Li-hua- JIN Yan
- FENG Xue-feng
- ZHANG Qi-wei
- SHEN Qiang
- LIU Xiao-qian
- ZHU Hui
- ZHANG Shan-shan
- WANG Zhi-min
- Institute of Chinese Materia Medica
- China Academy of Chinese Medical Sciences
- National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines
- National Resource Center for Chinese Materia Medica
- China Academy of Chinese Medical Sciences
- Changzhi Medical College