闫利华;金艳;冯学峰;张启伟;申强;刘晓谦;朱辉;张姗姗;王智民;
YAN Li-hua;JIN Yan;FENG Xue-feng;ZHANG Qi-wei;SHEN Qiang;LIU Xiao-qian;ZHU Hui;ZHANG Shan-shan;WANG Zhi-min;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Changzhi Medical College;

• 1:中国中医科学院中药研究所
• 2:中药质量控制技术国家工程实验室
• 3:中国中医科学院中药资源中心
• 4:长治医学院

摘要(Abstract)：

建立扶芳藤药材质量标准,为控制该药材的质量提供实验依据。采用经验、显微鉴别法对该药材性状、显微特征进行描述;参照2010年版《中国药典》附录相关方法,对该药材水分、总灰分、酸不溶性灰分、醇溶性浸出物进行测定;采用薄层色谱法,分别以卫矛醇和对照药材为对照,对该药材进行定性鉴别;在成分分布和组成分析的基础上,以苷元槲皮素和山柰素为指标,通过系统的方法学考察,建立了药材总黄酮醇苷的含量测定方法。含量测定方法采用C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.1%甲酸溶液(51∶49)为流动相,在366 nm波长下对槲皮素和山柰素进行定量分析,再根据换算系数计算总黄酮醇苷的含量。该药材性状、显微、薄层鉴别方法具有较强的专属性,水分平均为8.76%,总灰分平均为6.48%,酸不溶性灰分平均为0.31%,醇溶性浸出物平均为17.48%,总黄酮醇苷含量平均为0.211%。根据实验结果建立了扶芳藤药材质量标准,所建立的质量标准可用于扶芳藤药材的质量控制。
To establish quality standards of Euonymus fortunei,and supply scientific evidence for the quality control of Euonymus fortunei. Empirical and microscopic identification methods were adopted to observe morphological and histological characters. The contents of water,total ash,acid-insoluble ash and alcohol-soluble extractive were analysed according to the methods of Chinese Pharmacopoeia( 2010). Dulcitol and reference herbs were used to identify materia medica of Euonymus fortunei by TLC method. The total flavonol glycosides contents were analysed by HPLC method,using quercetin and kaempferol as reference substances. Quercetin and kaempferol were separated on a C18column( 4. 6 mm × 250 mm,5 μm) with methanol-0. 1% formic acid( 51∶ 49) as the mobile phase and detected at 366 nm. The flavonoid aglycones content was then multiplied by a conversion coefficient,and the result was the total flavonol glycosides content. The macroscopical identification,microscopic features and TLC methods were proper. The average contents of water,total ash,acid-insoluble ash,alcohol-soluble extractive and total flavonol glycosides were 8. 76%,6. 48%,0. 31%,17. 48% and 0. 211%,respectively. The quality standards established on the basis of the research results were suitable for the quality evaluation of Euonymus fortunei.

关键词(KeyWords)： 扶芳藤;质量标准;卫矛醇;槲皮素;山柰素;总黄酮醇苷
Euonymus fortunei;quality standards;dulcitol;quercetin;kaempferol;total flavonol glycosides

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2012ZX09304005002);; 国家药典委员会标准提高暨2015版《中国药典》科研任务

作者(Authors): 闫利华;金艳;冯学峰;张启伟;申强;刘晓谦;朱辉;张姗姗;王智民;
YAN Li-hua;JIN Yan;FENG Xue-feng;ZHANG Qi-wei;SHEN Qiang;LIU Xiao-qian;ZHU Hui;ZHANG Shan-shan;WANG Zhi-min;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Changzhi Medical College;

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