中国中药杂志

2020, v.45(04) 890-895

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丹参药渣等不同类型中药固废发酵产纤维素酶研究
Fermentation of cellulase with multiple types of Salvia miltiorrhiza residues and other solid wastes from Chinese materia medica industrialization

邱首哲;曾飞;张森;宿树兰;郭盛;钱大玮;赵明哲;段金廒;
QIU Shou-zhe;ZENG Fei;ZHANG Sen;SU Shu-lan;GUO Sheng;QIAN Da-wei;ZHAO Ming-zhe;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Key Laboratory of Chinese Medicinal Resources Recycling Utilization under State Administration of Traditional Chinese Medicine,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine;

摘要(Abstract):

以丹参药渣为代表的中药固废在生物转化高价值利用过程中,具有较强的小分子生物抗性屏障。选育高耐受性的功能转化菌株,破除丹参药渣的抗性屏障,产高附加值纤维素酶,对中药固废资源化与产业化利用具有科学价值和现实意义。该研究采用生物学方法,利用丹参药渣所含小分子抑菌物丹参酮优选产酶菌株,得到一株真菌扩展青霉SZ13。确定SZ13最优产酶工艺和产酶高峰期,发现SZ13在温度35℃,转速180 r·min~(-1),药渣添加量5%,种液接入量5%的条件下降解药渣,可以维持5 d的产酶高峰期。在此基础上,探究SZ13对不同类型药渣的降解产酶能力。结果显示,SZ13生物降解各类型药渣产酶的酶活高,稳定性强。SZ13可以高效利用丹参等不同类型中药渣等固体废弃物,实现不同类型药渣中纤维素的高值化利用。
The solid wastes of Chinese materia dedica industrialization represented by Salvia miltiorrhiza residues have a strong small-molecule bio-recalcitrance in the process of high-value utilization of biotransformation. Highly tolerant strains were bred to break bio-recalcitrance of Salvia miltiorrhiza residues and produce high-value added cellulose, which has a significant significance for recycling and industrial utilization of solid waste. In this study, a strain of fungus, Penicillium expansum SZ13, was found with small-molecule antibacterial substance tanshinone contained in Salvia miltiorrhiza residues by a biological method. The optimal enzyme production process and peak period of SZ13 were determined. It was found that SZ13 could maintain peak enzyme production for 5 days by degrading residues under the conditions of temperature 35 ℃, rotation speed 180 r·min~(-1), 5% of residues addition, and 5% seed solution addition. Meanwhile, the ability of SZ13 to degrade the enzyme production of multiple types of residues was explored. The results showed a high enzyme activity and stable enzyme production of SZ13 in the process of degrading residues. SZ13 could efficiently utilize various types of Chinese medicine residues, such as Salvia miltiorrhiza residues, to realize the high-value utilization of cellulose in multiple types of residues.

关键词(KeyWords): 中药固废物;丹参药渣;纤维素酶;扩展青霉;循环利用
solid waste from Chinese materia medica industrialization;Salvia miltiorrhiza residues;cellulase;Penicillium expansum;resources circulating utilization

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(81673533);; 江苏省高校自然科学研究面上项目(19KJ360017);; 江苏省自然科学基金青年项目(BK20161048,BK20161038)

作者(Author): 邱首哲;曾飞;张森;宿树兰;郭盛;钱大玮;赵明哲;段金廒;
QIU Shou-zhe;ZENG Fei;ZHANG Sen;SU Shu-lan;GUO Sheng;QIAN Da-wei;ZHAO Ming-zhe;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Key Laboratory of Chinese Medicinal Resources Recycling Utilization under State Administration of Traditional Chinese Medicine,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20191217.303

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