中国中药杂志

2011, v.36(17) 2358-2360

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来源于白木耳的诱导神经突起伸长的活性分子
A neuritogenic compound from Tremella fuciformis

张洋;裴亮;高丽娟;黄乾明;戚建华;
ZHANG Yang1,PEI Liang1,GAO Lijuan2,HUANG Qianming1,QI Jianhua2(1.College of Biology and Science,Sichuan Agricultural University,Yaan 625001,China;2.College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,China)

摘要(Abstract):

目的:发现具有类似神经生长因子(nerve growth factor,NGF)活性的小分子化合物。方法:通过生物活性实验筛选,发现白木耳的甲醇浸提物具有显著诱导PC12细胞发生神经突起伸长的作用。在生物活性鉴定实验的引导下,通过多次柱色谱分离,HPLC纯化,得到一纯净的活性化合物1,利用波谱分析法确定了其化学结构。结果:通过与文献的氢谱、碳谱、质谱对比,确定该活性分子属麦角甾醇苷类化合物,是3-O-β-D-glucopyranosyl-22E,24R-5α,8α-epidioxyergosta-6,22-diene。该化合物在3μmol.L-1浓度下具有显著诱导PC12细胞发生神经突起伸长的活性。结论:来源于白木耳的麦角甾醇苷类的化合物1具有显著的类似神经生长因子NGF的活性。
Objective: To search for low-molecular-weight neuritogenic compounds from the traditional Chinese medicine(TCM).Method: An extract library of TCM was prepared.Targeted isolation guided by biological screening led to the discovery of compound 1,and its structure was elucidated by analysis of spectroscopic methods and comparison of spectroscopic data with these re-ported from the literature.Result: A neuritogenic compound 1,3-O-β-D-glucopyranosyl-22E,24R-5α,8α-epidioxyergosta-6,22-diene,was isolated and identified from the methanol extract of T.fuciformis.This compound showed a significant neuritogenic activity against PC12 cells at 3 μmol.L-1.Conclusion: Methonal extract of T.fuciformis and targeted compound 1 both showed significant neuritogenic activity against PC12 cells.These results suggested that the extract and compound 1 might be used to prevent and treat neurodegenerative disease such as Alzheimer's disease.

关键词(KeyWords): 白木耳;神经生长因子NGF;PC12细胞;神经突起伸长;麦角甾醇苷类化合物
Tremella fuciformis;neuritogenic activity;PC12 cell;NGF mimics;3-O-β-D-glucopyranosyl-22E,24R-5α,8α-epidioxyergosta-6,22-diene

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作者(Authors): 张洋;裴亮;高丽娟;黄乾明;戚建华;
ZHANG Yang1,PEI Liang1,GAO Lijuan2,HUANG Qianming1,QI Jianhua2(1.College of Biology and Science,Sichuan Agricultural University,Yaan 625001,China;2.College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,China)

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