中国中药杂志

2012, v.37(19) 2964-2967

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HPLC-MS测定大鼠血浆中青蒿素浓度及其应用
Determination of artemisinin in rat plasma with HPLC-MS and its application

傅川;余继英;邹静;何林;董芸;黄园;
FU Chuan1,YU Jiying2,ZOU Jing2,HE Lin2,DONG Yun3,HUANG Yuan1(1.West China School of Pharmacy,Sichuan University,Chengdu 610041,China; 2.Department of Pharmacy,Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital,Chengdu 610072,China; 3.Intensive Care Unit Department,Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital,Chengdu 610072,China)

摘要(Abstract):

目的:建立青蒿素大鼠体内药物浓度的分析方法。方法:采用高效液相色谱-质谱联用技术,选用艾司唑仑为内标,样品与内标使用甲基叔丁基醚提取,并分别于m/z305,296进行测定。结果:在5~500μg.L-1,青蒿素峰面积与内标峰面积比值与浓度有良好的线性关系,最低定量质量浓度为5μg.L-1。结论:方法学证明该法能够满足青蒿素大鼠体内血药浓度的测定,可用于动物体内药物动力学的研究需求。
Objective:To develop a method for determining artemisinin in rat plasma in vivo.Method:HPLC-MS was adopted.Estazolam was selected as an internal standard(I.S.).The sample and I.S.were extracted using methyl tertbutyl ether and measured at m/z of 305 and 296,respectively.Result:Within the linear range of 5-500 μg·L-1,the ratio of artemisinin's peak area and I.S.peak area and the concentration showed good linearity,thus the minimum concentration was set to be 5 μg·L-1.Conclusion:The methodology proved that the method is so suitable for determining the drug concentration in rat blood that it can be used for studying pharmacokinetics in animals.

关键词(KeyWords): 青蒿素;高效液相色谱-质谱联用;药物动力学
artemisinin;HPLC-MS;pharmacokinetics

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作者(Author): 傅川;余继英;邹静;何林;董芸;黄园;
FU Chuan1,YU Jiying2,ZOU Jing2,HE Lin2,DONG Yun3,HUANG Yuan1(1.West China School of Pharmacy,Sichuan University,Chengdu 610041,China; 2.Department of Pharmacy,Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital,Chengdu 610072,China; 3.Intensive Care Unit Department,Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital,Chengdu 610072,China)

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